2023
Nwosu ZC*, Ward MH*, Sajjakulnukit P*, Poudel P*, Ragulan C, Kasperek S, Radyk M, Sutton D, Menjivar RE, Andren A, Saab JA, Tolstyka Z, Brown K, Lee HJ, Dzierozynski LN, He X, PS3 H, Ugras J, Nyamundanda G, Zhang L, Halbrook CJ, Carpenter ES, Shi J, Shriver LP, Patti GJ, Muir A, Pasca di Magliano M, Sadanandam A†, Lyssiotis CA†. Nutrient profiling reveals extracellular uridine as a fuel for pancreatic cancer through uridine phosphorylase 1. Nature (In Press) * – Co-first authors † – Corresponding authors
Halbrook CJ†, Pasca di Magliano M†, Lyssiotis CA†, Maitra A†. Pancreatic Cancer: Advances and Challenges (2023). Cell 186, 1729-1754, doi:10.1016/j.cell.2023.02.014 † – Corresponding authors
Menjivar RE, Nwosu ZC, Du W, Donahue KL, Hong HS, Espinoza C, Brown K, Velez-Delgado A, Yan W, Lima F, Bischoff A, Kadiyala P, Salas-Escabillas D, Crawford HC, Bednar F, Carpenter E, Zhang Y, Halbrook CJ, Lyssiotis CA, Pasca di Magliano M. Arginase 1 is a key driver of immune suppression in pancreatic cancer. Elife. 2023;12. doi: 10.7554/eLife.80721.
Beutel AK, Halbrook CJ. Barriers and Opportunities for Gemcitabine in Pancreatic Cancer Therapy. Am J Physio Cell Physiology. 2023;324(2):C540-C52. doi: 10.1152/ajpcell.00331.2022.
2022
Singha MK, Zimak J, Levine SR, Dai N, Hong C, Anaraki C, Gupta M, Halbrook CJ, Atwood SX, Spitale RC. An Optimized Enzyme-Nucleobase Pair Enables In Vivo RNA Metabolic Labeling with Improved Cell-Specificity. Biochemistry. 2022;61(23):2638-42. doi: 10.1021/acs.biochem.2c00559.
Halbrook CJ†, Thurston G, Boyer S, Anaraki C, Jiménez J, McCarthy A, Steele NG, Kerk SA, Hong H, Lin L, Law F, Felton C, Scipioni L, Sajjakulnukit P, Andren A, Beutel AK, Singh R, Nelson BS, Van Den Bergh F, Krall AS, Mullen PJ, Zhang L, Batra S, Morton JP, Stanger BZ, Christofk HR, Digman M, Beard DA, Viale A, Zhang J, Crawford HC, Pasca di Magliano M, Jorgensen C, Lyssiotis CA†. Differential integrated stress response and asparagine production drive symbiosis and therapy resistance of pancreatic adenocarcinoma cells. Nature Cancer 2022;3(11):1386-403. doi: 10.1038/s43018-022-00463-1. † – Corresponding authors
Kerk SA, Lin L, Myers A, Sutton DJ, Andren A, Sajjakulnukit P, Zhang L, Zhang Y, Jiménez JA, Nelson BS, Chen B, Robinson A, Thurston G, Kemp S, Steele NG, Hoffman MT, Wen HJ, Long D, Ackenhusen SE, Ramos J, Gao X, Nwosu ZC, Galbán S, Halbrook CJ, Lombard DB, Piwnica-Worms, Ying H, Crawford HC, Pasca di Magliano M, Shah YM†, Lyssiotis CA†. Metabolic requirement for GOT2 in pancreatic cancer depends on environmental context. eLife 2022 11:e73245 https://doi.org/10.7554/eLife.73245 † – Corresponding authors
Boyer SB, Lee HJ*, Steele NG, Zhang L, Sajjakulnukit P, Andren A, Basrur V, Zhang Y, Ward MH, Singh R, Nesvizhskii AI, Pasca di Magliano M, Halbrook CJ†, Lyssiotis, CA†. Multi-omic Characterization of Pancreatic Cancer-Associated Macrophage Polarization Reveals Deregulated Metabolic Programs Driven by the GMCSF-PI3K Pathway eLife 2022;11:e73796 DOI: 10.7554/eLife.73796 † – Corresponding authors
2021
Kim PK*, Halbrook CJ*, Kerk SA*, Radyk M, Wisner S, Kremer DM, Sajjakulnukit P, Andren A, Hou SW, Trivedi A, Thurston G, Anand A, Yan L, Salamanca-Cardona L, Welling SD, Zhang L, Pratt MR, Keshari KR, Ying H, Lyssiotis CA. Hyaluronic acid fuels pancreatic cancer cell growth (2021). eLife 2021;10:e62645. DOI: 10.7554/eLife.62645. * – Co-first Authors
Steele NG*, Biffi G*, Kemp SB, Zhang Y, Drouillard D, Syu L, Hao Y, Oni TE, Brosnan E, Elyada E, Doshi A, Hansma C, Espinoza C, Abbas A, The S, Irizarry-Negron V, Halbrook CJ, Franks NE, Hoffman MT, Brown K, Carpenter ES, Nwosu ZC, Johnson C, Lima F, Anderson MA, Park Y, Crawford HC, Lyssiotis CA, Frankel TL, Rao A, Bednar F, Dlugosz AA, Preall JB, Tuveson DA*, Allen BL*, Pasca di Magliano M*. Inhibition of Hedgehog Signaling Alters Fibroblast Composition in Pancreatic Cancer (2021). Clincal Cancer Reserch 27: 2023-2037
Krall AS*, Mullen PJ*, Surjono F, Momcilovic M, Schmid WE, Halbrook CJ, Thambundit A, Mittelman SD, Lyssiotis CA, Shackelford DB, Knott SRV, Christofk HR. Asparagine couples mitochondrial respiration to ATF4 activity and tumor growth. (2021) Cell Metabolism Epub 2021/02/21. doi: 10.1016/j.cmet.2021.02.001
2020
Zhou W, Yao Y, Scott A, Wilder-Romans K,Dresser J, Pratt D, Sajjakulnukit P, Halbrook CJ, Nelson BS, Sun H, Werner CK, Zhao SG, Davis M, Morgan MA, Rehemtualla A, Gatto F, Xiong J, Zhang L, Castro M, Lowenstein P, Chandrasekaran S, Lawrence TS, Lyssiotis CA, Wahl DR. De novo purine synthesis is a targetable vulnerability that regulates therapy resistance, DNA repair and survival in IDH-wild type glioblastoma (2020). Nature Comminucations 11(1):3811
Zhou Z*, Torres M*, Sha H, Halbrook CJ, Van den Bergh F, Reinert B, Yamada T, Wang S, Luo Y, Hunter A, Wang C, Sanderson TH, Lui M, Taylor A, Sesaki H, Lyssiotis CA, Wu J, Kersten S Beard D, Qi L. Regulation of endoplasmic reticulum-mitochondria contacts and mitochondrial dynamics by Sel1L-Hrd1 ERAD during thermogenesis (2020). Science Epub 2020/03/21. doi: 10.1126/science.aay2494. PubMed PMID: 32193362
Zhang Y, Lazarus J, Steele NG, Yan W, Lee HJ, Nwosu ZC, Halbrook CJ, Menjivar RE, Kemp SB, Sirihorachai V, Velez-Delgado A, Donahue K, Carpenter ES, Brown KL, Irizarry-Negron V, Nevison AC, Vinta A, Anderson MA, Crawford HC, Lyssiotis CA, Frankel TL, Bednar F, Pasca di Magliano M. Regulatory T cell depletion causes compensatory immune suppression and accelerated pancreatic carcinogenesis (2020). Cancer Discovery 0(3):422-39. Epub 2020/01/09. doi: 10.1158/2159-8290.CD-19-0958.
Lanfranca MP, Zhang Y, Girgis A, Kasselman S, Lazarus J, Kryczek I, Delrosario L, Rhim A, Koneva L, Sartor M, Sun L, Halbrook CJ, Nathan H, Shi J, Crawford HC, Pasca di Magliano M, Zou W, Frankel TL. Interleukin 22 Signaling Regulates Acinar Cell Plasticity to Promote Pancreatic Tumor Development in Mice (2020). Gastroenterology. 2019. Epub 2019/12/18. doi:10.1053/j.gastro.2019.12.010.
Nelson BS, Lin L, Kremer DM, Sousa CM, Cotta-Ramusino C, Myers A, Ramos J, Gao T, Kovalenko I, Wilder-Romans K, Dresser J, Davis M, Nwosu Z, Campit S, Mashadova O, Nicolay B, Tolstyka Z, Halbrook CJ, Chandrasekaran S, Asara JM, Crawford HC, Cantley LC, Kimmelman AC, Wahl DR, Lyssiotis CA. Tissue of Origin Dictates GOT1 Dependence and Confers Synthetic Lethality to Radiotherapy (2020). Cancer and Metabolism Jan 2;8:1. doi: 10.1186/s40170-019-0202-2
2019
Halbrook CJ, Crawford HC (2019). Hiding in Plain Sight. Science 364, 1132-1133
Walter LA, Lin YH, Halbrook CJ, Chuh KN, He L, Pedowitz NJ, Batt AR, Brennan CK, Stiles BL, Lyssiotis CA, Pratt MR. Inhibiting the biosynthesis of UDP-GlcNAc lowers O-GlcNAcylated levels and sensitizes cancer to environmental stress (2019). Biochemistry. Epub 2019/10/19. doi: 10.1021/acs.biochem.9b00560.
Halbrook CJ, Nelson BS, Lyssiotis CA (2019). Metabolism Drives Carcinogenesis and Maintenance of Pancreatic Tumors. Cancer Discovery. 9:326-328
Wang L, Yang H, Zamparone A, Diolaiti D, Palmbos P, Abel E, Purohit V, Dolgalev I, Rhim A, Ljungman M, Hadju C, Halbrook CJ, Bar-Sagi D, Pasca di Magliano M, Crawford H, Simeone D (2019). ATDC is required for the initiation of Kras-induced tumorigenesis. Genes Dev 33, 641-655
Halbrook CJ, Pontious C, Lee HJ, Kovalenko I, Zhang Y, Lapienyte L, Dreyer S, Sajjakulnukit P, Kremer DM, Zhang L, Nelson B, Hong H, Kemp S, Crawford HC, Bianken A, Chang D, Morton JP, Pasca di Magliano M, Costas A. Lyssiotis. Macrophage Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer (2019). Cell Metabolism – 29, 1390-1399 e1396
Halbrook CJ, Wahl DR, Lyssiotis CA (2019). Running the Light: Nucleotide Metabolism Drives Bypass of Senescence in Cancer. Trends in Biochem Sci 44(12):991-993
2018
Svoboda LK, The SS, Kerk S, Sud S, Zebolsky A, Treichel S, Thomas D, Halbrook CJ, Lee H-J, Kremer DL, Zhang L, Magnuson B, Bankhead A, Ljungman M, Cierpicki T, Grembecka J, Lyssiotis CA, Lawlor ER (2018). Regulation of the serine biosynthetic pathway by the menin-MLL complex in Ewing sarcoma. J Pathol. 245(3):324-336
Schofield HK, Zeller J, Espinoza C, Halbrook CJ, del Vecchio A, Magnuson BR, Fabo T, Daylan A, Kovalenko I, Lee H, Yan W, Feng Y, Karim SA, Kremer DM, Kumar-Sinha C, Lyssiotis CA, Ljungman M, Morton JP, Galban S, Fearon ER, Pasca di Magliano M (2018). Mutant p53R270H drives altered metabolism and increased invasion in pancreatic ductal adenocarcinoma. JCI Insight 3 (2)
Halbrook CJ, Nwosu ZC, Lyssiotis CA (2018). Fine TUNE-ing mitochondrial dysfunction and reductive carboxylation. Trends Endocrinol Metab.;29(9):599-602.
Schofield HK, Tandon M, Park MJ, Halbrook CJ, Ramakrishnan SK, Kim EC, Shi J, Omary MB, Shah YM, Esni F, Pasca di Magliano M. Pancreatic HIF2α stabilization leads to chronic pancreatitis and predisposes to mucinous cystic neoplasm (2018). Cell Mol Gastroenterol Hepatol 5, 169-185.
Perusina-Lanfranca M, Thompson JK, Bednar F, Halbrook CJ, Lyssiotis CA, Levi B, Frankel TL. Metabolism and Epigenetics of Pancreatic Cancer Stem Cells (2018). Semin Cancer Biol. pii: S1044-579X(18)30116-0.
Halbrook CJ, Pasca di Magliano MP, Lyssiotis CA (2018). “Tumor Crosstalk Networks Promote Growth and Support Survival of Pancreatic Cancer” Am J Physiol Gastrointest Liver Physiol. 315(1):G27-G35
2017
Halbrook CJ, Lyssiotis, CA (2017). Employing metabolism to improve the diagnosis and treatment of pancreatic cancer. Cancer Cell 31, 5-19
Halbrook CJ, Wen HJ, Ruggeri J, Takeuchi KK, Zhang Y, Pasca di Magliano M, Crawford HC. Mitogen-activated Protein Kinase Kinase Activity Maintains Acinar-to-Ductal Metaplasia and Is Required for Organ Regeneration in Pancreatitis (2017). Cell Mol Gastroenterol Hepatol 3, 99-118
2016
Roy N*, Takeuchi KK*, Ruggeri JM, Bailey P, Chang D, Li J, Leonhardt L, Puri S, Hoffman MT, Gao S, Halbrook CJ, Song Y, Ljungman M, Malik S, Wright CV, Dawson DW, Biankin AV, Hebrok M, and Crawford HC (2016). PDX1 dynamically regulates pancreatic ductal adenocarcinoma initiation and maintenance. Genes Dev 30(24): 2669-2683
Sousa CM, Biancur DE, Wang X, Halbrook CJ, Sherman MH, Zhang L, Kremer D, Hwang RF, Witkiewicz AK, Ying H, Asara JM, Evans RM, Cantley LC, Lyssiotis CA, Kimmelman AC (2016). Pancreatic tumor-associated stellate cells support cancer cell metabolism through autophagy-dependent alanine secretion. Nature 536, 479-483, doi:10.1038/nature19084
2014
Wu CY*, Carpenter ES*, Takeuchi KK*, Halbrook CJ, Peverley LV, Bien H, Hall JC, DelGiorno KE, Pal D, Song Y, Shi C, Liz RZ, Crawford, HC (2014). PI3K Regulation of RAC1 is required for KRAS-induced pancreatic tumorigenesis in mice. Gastroenterology 147, 1405-1416 e1407.
Delgiorno KE, Hall JC, Takeuchi KK, Pan FC, Halbrook, CJ, Washington MK, Olive KP, Spence JR, Sipos B, Wright CV, Wells JM, Crawford HC (2014). Identification and manipulation of biliary metaplasia in pancreatic tumors. Gastroenterology 146, 233-244 e235.
2012
Ardito CM*, Gruner BM*, Takeuchi KK, Lubeseder-Martellato C, Teichmann N, Mazur PK, Delgiorno KE, Carpenter ES, Halbrook CJ, Hall JC, Pal D, Briel T, Herner A, Trajkovic-Arsic M, Sipos B, Liou GY, Storz P, Murray NR, Threadgill DW, Sibilia M, Washington MK, Wilson CL, Schmid RM, Raines EW, Crawford HC*, Siveke JT* (2012). EGF receptor is required for KRAS-induced pancreatic tumorigenesis. Cancer Cell 22, 304-317.