Publications

 

SELECTED PUBLICATIONS

– 2024 –

Lee CC, Stang M, Ribbe MW, Hu Y (2024). ATP-independent turnover of dinitrogen intermediates captured on the nitrogenase cofactor. Angew Chem Int Ed Engl 2024 Mar 25:e202400273. DOI: 10.1002/anie.202400273.

 

– 2023 –

Quechol R, Solomon JB, Liu YA, Lee CC, Jasniewski AJ, Górecki K, Oyala P, Hedman B, Hodgson KO, Ribbe MW, Hu Y (2023). Heterologous synthesis of the complex homometallic cores of nitrogenase P- and M-clusters in Escherichia coli. Proc Natl Acad Sci USA, 120(44):e2314788120.

Hu Y, Lee CC, Grosch M, Solomon JB, Weigand W, Ribbe MW (2023). Enzymatic Fischer-Tropsch-Type Reactions. Chem Rev, 123(9):5755-5797.

Ribbe MW, Hu Y (2023). Belt sulfur mobilization in nitrogenase biosynthesis and catalysis. Trends Chem, 5(2): 108-111.

 

– 2022 –

Stripp ST, Duffus BR, Fourmond V, Léger C, Leimkühler S, Hirota S, Hu Y, Jasniewski A, Ogata H, Ribbe MW (2022). Second and outer coordination sphere effects in low valent metalloenzymes. Chem Rev, 122(14):11900-11973.

Lee CC, Kang W, Jasniewski AJ, Stiebritz MT, Tanifuji K, Ribbe MW, Hu Y (2022). Evidence of substrate binding and product release via belt-sulphur mobilization of the nitrogenase cofactor. Nature Catalysis, 5:443-454.

Solomon JB, Tanifuji K, Lee CC, Jasniewski AJ, Hedman B, Hodgson KO, Hu Y, Ribbe MW (2022). Characterization of a nitrogenase iron protein substituted with a synthetic [Fe4Se4] cluster. Angew Chem Int Ed Engl, 61(19):e202202271.

 

– 2021 –

Tanifuji K, Jasniewski AJ, Villarreal D, Stiebritz MT, Lee CC, Wilcoxen J, Okhi Y, Chatterjee R, Bogacz I, Yano J, Kern J, Hedman B, Hodgson KO, Britt RD, Hu Y, Ribbe MW (2021). Tracing the incorporation of the 9th sulfur into the nitrogenase cofactor precursor with selenite and tellurite. Nature Chemistry, 13(12):1228-1234.

Solomon JB, Rasekh MF, Hiller CJ, Lee CC, Tanifuji K, Ribbe MW, Hu Y (2021) Probing the all-ferrous states of methanogen nitrogenase iron proteins. JACS Au, 1:119–123.

Kang W, Rettberg LA, Stiebritz M, Jasniewski AJ, Tanifuji K, Lee CC, Ribbe MW, Hu Y (2021) Crystallographic analysis of NifB with a full complement of clusters: Structural insights into the radical SAM-dependent carbide insertion during nitrogenase cofactor assembly. Angew Chem Int Ed Engl, 60(5):2364-2370.

 

– 2020 –

Kang, W., Lee, C.C., Jasniewski, A.J., Ribbe, M.W. & Hu, Y. (2020) Structural evidence for a dynamic metallocofactor during N2 reduction by Mo-nitrogenase. Science, 368, 1381-1385.

Rettberg, L.A., Wilcoxen, J., Jasniewski, A.J., Lee, C.C., Tanifuji, K., Hu, Y., Britt, R.D. & Ribbe, M.W. (2020) Identity and function of an essential nitrogen ligand of the nitrogenase cofactor biosynthesis protein NifB. Nature Commun., 11, 1757.

Solomon, J.B., Lee, C.C., Jasniewski, A.J., Rasekh, M.F., Ribbe, M.W. & Hu, Y. (2020) .Heterologous expression and engineering of the nitrogenase cofactor biosynthesis scaffold NifEN. Angew. Chem. Int. Ed. Engl. 59, 6887-6893.

Jasniewski, A., Lee, C.C., Ribbe, M.W. & Hu, Y. (2020) Reactivity, mechanism, and assembly of the alternative nitrogenases. Chem. Rev., 120, 5107-5157.

Ribbe, M.W. (2020) Author Profile Markus W Ribbe. Angew. Chem. Int. Ed. Engl., 59, 10695.

Hu, Y. (2020) Author Profile: Yilin Hu. Angew. Chem. Int. Ed. Engl., 59, 10694.

 

– 2019 –

Jasniewski, A.J., Wilcoxen, J., Tanifuji, K., Hedman, B., Hodgson, K.O., Britt, R.D., Hu ,Y. & Ribbe, M.W. (2019). Spectroscopic characterization of an eight-iron nitrogenase cofactor precursor that lacks the “9th sulfur”. Angew. Chem. Int. Ed. Engl., 58, 14703-1470.

Lee, C. C., Stiebritz, M. T. & Hu, Y. (2019) Reactivity of [Fe4S4] clusters toward C1 substrates: mechanism, implications and potential applications. Acc. Chem. Res., 52, 1168-1176.

 

– 2018 –

Tanifuji, K., Lee, C.C., Sickerman, N.S., Tatsumi, K., Ohki, Y., Hu, Y. & Ribbe, M.W. (2018). Tracing the ‘9th Sulfur’ of the nitrogenase cofactor via a semi-synthetic approach. Nature Chem., 10, 568-572

Lee, C.C., Hu, Y. & Ribbe, M.W. (2018). Reduction and condensation of aldehydes by the isolated cofactor of nitrogenase. ACS Cent. Sci., 4, 1430-1435.

Rettberg, L.A., Wilcoxen, J., Lee, C.C., Stiebritz, M.T., Tanifuji, K., Britt, R.D. & Hu, Y. (2018) Probing the coordination and function of Fe4S4 modules in nitrogenase assembly protein NifB. Nature Commun., 9, 2824.

Stiebritz, M.T., Hiller, C.J., Sickerman, N.S., Lee, C.C., Tanifuji, K., Ohki, Y. & Hu, Y. (2018) Ambient conversion of CO2 to hydrocarbons by biogenic and synthetic [Fe4S4] clusters. Nature Catal., 1, 444–451.

Lee, C.C., Wilcoxen, J., Hiller, C.J., Britt, R.D. & Hu, Y. (2018) Evaluation of the catalytic relevance of the CO-bound states of V-nitrogenase. Angew. Chem. Int. Ed. Engl., 57, 3411-3414.

 

– 2017 –

Sickerman, N.S., Ribbe, M.W. & Hu, Y. (2017). Nitrogenase Cofactor Assembly: An Elemental Inventory. Acc. Chem. Res., 50, 2834-2841.

Sickerman, N.S., Tanifuji, K., Lee, L.L., Ohki, Y., Tatsumi, K., Ribbe, M.W. & Hu, Y. (2017). Reduction of C1 substrates to hydrocarbons by the homometallic precursor and synthetic mimic of the nitrogenase cofactor. J. Am. Chem. Soc., 139, 603-606.

Rebelein, J.G., Stiebritz, M.T., Lee, C.C. & Hu, Y. (2017) Activation and reduction of carbon dioxide by nitrogenase iron proteins. Nature Chem. Biol., 13, 147-149

 

– 2016 –

Rebelein, J.G., Lee, C.C., Hu, Y. & Ribbe, M.W. (2016). The in vivo Hydrocarbon Formation by Vanadium Nitrogenase Follows a Secondary Metabolic Pathway. Nature Commun., 7, 13641.

Tanifuji, K., Sickerman, N.S., Lee, C.C., Nagasawa, T., Miyazaki, K., Ohki, Y., Tatsumi, K., Hu, Y. & Ribbe, M.W. (2016) Structure and Reactivity of an Asymmetric Synthetic Mimic of Nitrogenase Cofactor. Angew. Chem. Int. Ed. Engl., 55, 15633-15636.

Fay, A. W., Blank, M. A., Rebelein, J. G., Lee, C. C., Ribbe, M. W., Hedman, B., Hodgson, K. O. & Hu, Y. (2016). Assembly scaffold NifEN: A structural and functional homolog of the nitrogenase catalytic component. Proc. Natl. Acad. Sci. USA, 113, 9504-9508.

Hu, Y. & Ribbe, M.W. (2016). Nitrogenases – A tale of carbon atom(s). Angew. Chem. Int. Ed. Engl., 55, 8216-8226.

Hu, Y. & Ribbe, M.W. (2016). Biosynthesis of the Metalloclusters of Nitrogenases. Annu. Rev. Biochem., 85, 455-483.

 

– 2015 –

Fay, A.W., Wiig, J.A., Lee, C.C. & Hu, Y. (2015) Identification and characterization of functional homologs of nitrogenase cofactor biosynthesis protein NifB from methanogens. Proc. Natl. Acad. Sci. USA, 112, 14829-14833.

Lee, C.C., Fay, A.W., Weng, T.C., Krest, C.M., Hedman, B., Hodgson, K.O., Hu, Y. & Ribbe, M.W. (2015). Uncoupling binding of substrate CO from turnover by vanadium nitrogenase. Proc. Natl. Acad. Sci. USA, 112, 13845-13849.

Tanifuji, K., Lee, C. C., Ohki, Y., Tatsumi, K., Hu, Y. & Ribbe, M.W. (2015). Combining a Nitrogenase Scaffold and a Synthetic Compound into an Artificial Enzyme. Angew. Chem. Int. Ed. Engl., 54, 14022-14025.

Wiig, J.A., Hu, Y. & Ribbe, M.W. (2015). Refining the pathway of carbide insertion into the nitrogenase M-cluster. Nature Commun., 6, 8034.

Ribbe, M.W. (2015). Insights into the mechanism of carbon monoxide dehydrogenase at atomic resolution. Angew. Chem. Int. Ed. Engl., 54, 8337-8339.

Lee, C.C., Hu, Y. & Ribbe, M.W. (2015). Catalytic reduction of CN–, CO and CO2 by nitrogenase cofactors in lanthanide-driven reactions. Angew. Chem. Int. Ed. Engl., 54, 1219-1222.

 

– 2014 –

Ribbe, M.W., Hu, Y., Hodgson, K.O., Hedman, B. (2014). Biosynthesis of nitrogenase metalloclusters. Chem. Rev., 114, 4063-4080.

Rebelein, J.G., Hu, Y. & Ribbe, M.W. (2014). Reduction of CO2 by nitrogenases. Angew. Chem. Int. Ed. Engl., 53, 11543-11546.

 

– 2013 –

Lancaster, K. M., Hu, Y., Bergmann, U., Ribbe, M. W. & Debeer, S. (2013). X-ray spectroscopic observation of an interstitial carbide in NifEN-bound FeMoco precursor. J. Am. Chem. Soc., 136, 610-612.

Moser, J., Lange, C., Krausze, J., Rebelein, J., Schubert, W. D., Ribbe, M., Heinz, D. W. & Jahn, D. (2013). Structure of ADP•AlF3-stabilized protochlorophyllide oxidoreductase complex. Proc. Natl. Acad. Sci. USA., 110, 2094-2098.

Wiig, J. A., Lee, C. C., Hu, Y. & Ribbe, M. W. (2013). Tracing the Interstitial Carbide of the Nitrogenase Cofactor during Substrate Turnover. J. Am. Chem. Soc., 135, 4982-4983.

Ni, F., Lee, C.C., Hwang, C.S., Hu, Y., Ribbe, M.W. & McKenna, C.E. (2013). Reduction of Fluorinated Cyclopropene by Nitrogenase. J. Am. Chem. Soc., 135, 10346-10352.

 

– 2012 –

Lee, C. C., Hu, Y. & Ribbe, M. W. (2012). ATP-Independent hydrocarbon formation catalyzed by isolated nitrogenase cofactors. Angew. Chem. Int. Ed. Engl., 51, 1947-1949.

Lee, C. C., Hu, Y. & Ribbe, M. W. (2012). ATP-Independent substrate reduction by nitrogenase P-cluster variant. Proc. Natl. Acad. Sci. USA, 109, 6922-6926.

Rupnik, K. S., Hu, Y., Lee, C. C., Wiig, J. A., Ribbe, M. W. & Hales, B. J. (2012). P+ State of nitrogenase P-cluster exhibits electronic structure of a [Fe4S4]+ cluster. J. Am. Chem. Soc., 134, 13749-13754.

Wiig, J. A., Hu, Y., Lee, C. C. & Ribbe, M. W. (2012). Radical SAM-dependent carbon insertion into the nitrogenase M-cluster. Science, 337, 1672-1675.

 

– 2011 –

Kaiser, J. T., Hu, Y., Wiig, J. A., Rees, D. C. & Ribbe, M. W. (2011). Structure of precursor bound NifEN: A nitrogenase FeMo cofactor maturase/insertase. Science, 331, 91-94.

Rupnik, K., Lee, C. C., Hu, Y., Ribbe, M. W. & Hales, B. J. (2011). [4Fe4S]2+ Clusters exhibit ground-state paramagnetism. J. Am. Chem. Soc., 133, 6871-6873.

Lee, C. C., Hu, Y. & Ribbe, M. W. (2011). Tracing the hydrogen source of hydrocarbons formed by vanadium nitrogenase. Angew. Chem. Int. Ed. Engl., 50, 5545-5547.

Wiig, J. A., Hu, Y. & Ribbe, M. W. (2011). NifEN-B complex of Azotobacter vinelandii is fully functional in nitrogenase FeMo cofactor assembly. Proc. Natl. Acad. Sci. USA, 108, 8623-8627.

Fay, A. W., Blank, M. A., Lee, C. C., Hu, Y., Hodgson, K. O., Hedman, B. & Ribbe, M. W. (2011). Spectroscopic characterization of isolated FeMoco precursor from NifEN. Angew. Chem. Int. Ed. Engl., 50, 7123-7128.

Hu, Y., Lee, C. C. & Ribbe, M. W. (2011). Extending the carbon chain: Hydrocarbon formation catalyzed by vanadium/molybdenum nitrogenases. Science, 333, 753-755.

Lancaster, K. M., Roemelt, M., Ettenhuber, P., Hu, Y., Ribbe, M. W., Neese, F., Bergmann, U. & DeBeer, S. (2011). Evidence for an interstitial carbide atom in the FeMo cofactor of nitrogenase from valence to core X-ray emission spectroscopy. Science, 334, 974-977.

 

– 2010 –

Hu, Y. & Ribbe, M. W. (2010). Decoding nitrogenase mechanism: The homolog approach. Acc. Chem. Res., 43, 475-484.

Lee, C. C., Hu, Y. & Ribbe, M. W. (2010). Vanadium nitrogenase reduces CO. Science, 329, 642.

Fay, A. W., Blank, M. A., Lee, C. C., Hu, Y., Hodgson, K. O., Hedman, B. & Ribbe, M. W. (2010). Characterization of isolated nitrogenase FeVco. J. Am. Chem. Soc., 132, 12612-12618.

 

– 2009 –

Cotton, M. S., Rupnik, K., Broach, R. B., Hu, Y., Fay, A. W., Ribbe, M. W. & Hales, B. J. (2009). VTVH-MCD study of the ∆nifB∆nifZ MoFe protein from Azotobacter vinelandii.  J. Am. Chem. Soc., 131, 4558-4559.

Lee, C. C., Hu, Y. & Ribbe, M. W. (2009). Unique features of the nitrogenase VFe protein from Azotobacter vinelandii. Proc. Natl. Acad. Sci. USA, 106, 9209-9214.

Yoshizawa, J. M., Blank, M. A., Fay, A. W., Lee, C. C., Wiig, J. A., Hu, Y., Hodgson, K. O., Hedman, B. & Ribbe, M. W. (2009). Optimization of FeMoco maturation on NifEN. J. Am. Chem. Soc., 131, 9321-9325.

Hu, Y., Yoshizawa, J. M., Fay, A. W., Lee, C. C., Wiig, J. A. & Ribbe, M. W. (2009). Catalytic activities of NifEN. Proc. Natl. Acad. Sci. USA, 106, 16962-16966.

Lee, C. C., Blank, M. A., Fay, A. W., Yoshizawa, J. M., Hu, Y., Hodgson, K. O., Hedman, B. & Ribbe, M. W. (2009). Stepwise formation of P-cluster in nitrogenase MoFe protein. Proc. Natl. Acad. Sci. USA, 106, 18474-18478.

Schwarz, G., Mendel, R. R. & Ribbe, M. W. (2009). Molybdenum cofactors, enzymes and pathways. Nature, 460, 839-847.

 

– 2008 –

Hu, Y., Fay, A. W., Lee, C. C., Yoshizawa, J. & Ribbe M. W. (2008). Assembly of nitrogenase MoFe protein. Biochemistry, 47, 3973-3981.

 

– 2007 –

Hu, Y., Fay, A. W., Lee, C. C. & Ribbe, M. W. (2007). P-Cluster maturation on nitrogenase MoFe protein. Proc. Natl. Acad. Sci. USA, 104, 10424-10429.

 

– 2006 –

Corbett, M. C., Hu, Y., Fay, A. W., Ribbe, M. W., Hedman, B. & Hodgson, K. O. (2006). Structural analysis of a protein-bound FeMo cofactor precursor. Proc. Natl. Acad. Sci. USA, 103, 1238-1243.

Hu, Y., Corbett, M. C., Fay, A. W., Webber, J. A., Hodgson, K. O., Hedman, B. & Ribbe, M. W. (2006). FeMo cofactor maturation on NifEN. Proc. Natl. Acad. Sci. USA, 103, 17119-17124.

Hu, Y., Corbett, M. C., Fay, A. W., Webber, J. A., Hodgson, K. O., Hedman, B. & Ribbe, M. W. (2006). Nitrogenase Fe protein: A molybdate/homocitrate insertase. Proc. Natl. Acad. Sci. USA, 103, 17125-17130.

 

– 2005 –

Hu, Y., Fay, A. W. & Ribbe, M. W. (2005). Identification of a nitrogenase iron-molybdenum cofactor precursor on NifEN complex. Proc. Natl. Acad. Sci. USA, 102, 3236-3241.

Hu, Y., Corbett, M. C., Fay, A. W., Webber, J. A., Hedman, B., Hodgson, K. O. & Ribbe, M. W. (2005). Nitrogenase reactivity with P-cluster variants. Proc. Natl. Acad. Sci. USA, 102, 13825-13830.

 

– 2002 –

Schmid, B., Ribbe, M. W., Einsle, O., Yoshida, M., Thomas, L. M., Dean, D. R., Rees, D. C. & Burgess, B. K. (2002). Structure of a cofactor-deficient nitrogenase MoFe protein. Science, 296, 352-356.