CYCLONE PHOSPHOR IMAGER
Phosphor film is loaded onto a drum inside the imager which is scanned to produce an image.
CASSETTE WITH PHOSPHOR FILM AND SLIDES
Phosphor film shown in a cassette. Slides (facing down) are loaded on to the film and the cassette closed. Exposure duration depends on the radioisotope under study.
OPTIQUANT SOFTWARE
Screen shot of the Optiquant software which is used to quantitatvely analyze the autoradiograms. Example above shows a tritium-labeled brain slice (3H-PIB, binding to amyloid plaques). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5569313/
IN VITRO AUTORADIOGRAPHY
Brain
Figure 18F-Nifene: Horizontal brain slices of rat brain showing binding of 18F-nifene (red = highest binding and white = lowest binding). (a). total binding (cor: frontal cortex; str: striatum; avt: anterioventral thalamus; th: thalamus; ps: pre/parasubiculum; cb: cerebellum). (b). same slice of total binding as in (a) with enhanced intensity (x2.5) to show extrathalamic regions. (c). binding in the presence of 10 nM nicotine showing partial displacement of 18F-nifene. (d). binding in the presence of 300 mM nicotine showing >90% displacemnt of 18F-nifene. (e). quantitation of the binding of 18F-nifene in brain regions from (b), (c) and (d). AVT exhibited the highest binding as evidenced by the region in red within the thalamus in (a).
https://pubmed.ncbi.nlm.nih.gov/16631077/
Brown Adipose Tissue
Figure 18F-FDG IBAT. Ex-vivo 18F-FDG autoradiography of BAT (upper row) showing intense uptake in the CL316,243-activated IBAT (right) as compared to control (left). Consecutive sections of autoradiographic images (Lower row) showing IBAT tissue marked by arrows (a). Hematoxylin-Eosin stained sections of control IBAT shows multiocular cells (×200 original magnification) which are disappeared when it gets activated by CL316,243 (b). Quantification of the Hematoxylin-Eosin stained sections in both groups (c). Data are Mean±SD; P<0.004 as compared to control* https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3250993/
EX VIVO AUTORADIOGRAPHY
Figure 18F-Mefway (A-D). Ex vivo horizontal brain slices of rodent brain showing binding of [18F]mefway (red = highest binding and white = lowest binding; OL=outer layer of cortex; ML= middle layer of cortex; IL=inner layer of cortex; ACC=anterior cingulated cortex; DG= dentate gyrus; iEC= inner enthorinal cortex; oEC= outer enthorinal cortex; LS= lateral septal nuclei; DRN= dorsal raphe nucleus; CA1, CA2, CA3= hippocampal regions). (E). Quantitation of [18F]mefway binding in hippocampal regions, dorsal raphe and septal nuclei in ex vivo autoradiographs and ratio with respect to the cerebellum; (F). Quantitation of [18F]mefway binding in cortical layers in ex vivo autoradiographs and ratio with respect to cerebellum. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3744326/
IODINE-125 AUTORADIOGRAPHY
Figure 125i-IPPI: Tau in human anterior cingulate (AC) and corpus callosum (CC): (A). Anti-Tau IHC showing NFT in the gray matter (GM) regions with little or no staining in white matter (WM) regions; (B-D). Pixel thresholder and pixel classifier identifies all Tau positivity in GM and WM regions; (E.). [125I]IPPI Tau, DLU/mm2 in GM and WM regions; (F). Correlation plot of [125I]IPPI per mm2 with % Tau positivity.
https://www.mdpi.com/2227-9059/11/4/1033
EVALUATING EFFECT OF MAGNETIC FIELD ON POSITRONS USING AUTORADIOGRAPHY
