Links and Training Resources

Multiphoton Excitation  Spectra of Fluorochromes

Two-photon absorption properties of fluorescent proteins https://pubmed.ncbi.nlm.nih.gov/21527931/

Excitation spectra and brightness optimization of two-photon excited probes https://pubmed.ncbi.nlm.nih.gov/22385865/

https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/technical-notes-and-product-highlights/fluorescent-probes-for-two-photon-microscopy.html

Salsa6f Resources

LSL-Salsa6f Transgenic Reporter Mice (JAX 031968) https://www.jax.org/strain/031968 

Salsa6f Plasmid (Addgne #140188) https://www.addgene.org/140188/

T-cell preparation for live-cell imaging using Salsa6f In Vitro https://bio-protocol.org/prep917 

Spectrum Viewers

https://www.fpbase.org/

https://www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer

https://www.thermofisher.com/order/fluorescence-spectraviewer#!/

https://searchlight.semrock.com/

Gene expression databases

BIOGPS: http://biogps.org/#goto=welcome

Immunological Genome Project: https://www.immgen.org/Databrowser19/DatabrowserPage.html http://rstats.immgen.org/Skyline/skyline.html

Brain Atlas

https://portal.brain-map.org/#

Brain RNA-Seq

https://www.brainrnaseq.org/

UCI FLOW CYTOMETRY

Flow Cytometry Services

https://cpb-us-e2.wpmucdn.com/sites.uci.edu/dist/e/3832/files/2020/10/IFI-Instrument-configurations_2020.pdf

Collaborations

• The Cahalan Lab http://crt.biomol.uci.edu/
• The Marangoni Lab https://www.immunoimaging.org/
• The Wagar Lab https://wagarlab.webflow.io/
• The Loaden Lab https://lodoenlab.bio.uci.edu/
• The Lane Lab https://faculty.sites.uci.edu/lanelab/
• The Pathak Lab http://www.pathaklab.org/
• The Acharya Lab https://www.faculty.uci.edu/profile.cfm?faculty_id=7045
• The Parker Lab https://parkerlab.bio.uci.edu/index.htm
• VRDC https://vaccine.uci.edu/research/ 
• IFI https://immunology.uci.edu/

UCI Links

• Department of Physiology and Biophysics, UCI
  http://www.physiology.uci.edu/
• UCI Institute for Immunology
  http://www.immunology.uci.edu/
• UCI Transgenic Mouse Facility
  https://www.research.uci.edu/facilities-services/tmf/index.html
• UCI Genomics High Throughput Facility  https://ghtf.biochem.uci.edu/

Immunology Courses

iBiology Immunology Course              https://www.youtube.com/watch?v=QT1FhFKLLhU&list=PLXwMdtHQav9bwkYjX1frWSlaO7cN7WLSW

Microscopy Courses

iBiology Short Microscopy Course  https://www.youtube.com/playlist?list=PLQFc-Dxlf4pSRaEk8Xi9BzS0r8-LYmwRQhttps://www.ibiology.org/online-biology-courses/short-microscopy-series/

iBiology Full Microscopy Course   https://www.youtube.com/playlist?list=PLQFc-Dxlf4pSHREZvz41xHFSEp65iNkBL

iBiology Flow Cytometry Course    https://www.youtube.com/watch?v=W1BFeiDwqnk&list=PLQFc-Dxlf4pSWdxEFTgOMkJhtlkq3m4mP 

Microscopy Resources

https://micro.magnet.fsu.edu/

https://www.microscopyu.com/

https://zeiss-campus.magnet.fsu.edu/index.html/

https://calm.ucsf.edu/resources

Image Analysis 

Microscopy: Image Analysis (Kurt Thorn)  https://www.youtube.com/watch?v=jaY6S1p4i3A

Microscopy: Introduction to Digital Images (Kurt Thorn)  https://www.youtube.com/watch?v=SNl3kRBgW10

Bioimage Analysis – Christian Tischer (EMBL)  https://www.youtube.com/watch?v=0PP38Z0CNMI

Imaris 

Introduction to Image Analysis using Imaris  https://www.youtube.com/watch?v=VqFh2Up5K7M

Introduction to Time Lapse in Imaris  https://imaris.oxinst.com/learning/view/article/introduction-to-time-lapse-in-imaris

Become a Master of Tracking in Imaris  https://imaris.oxinst.com/learning/view/article/mastering-tracking-in-imaris-3d-4d-visualization

Imaris Homeschool Webinars

https://imaris.oxinst.com/homeschool

https://www.youtube.com/watch?v=kC8bNWKyHvc

https://imaris.oxinst.com/learning/view/article/introduction-to-image-analysis-using-imaris

https://imaris.oxinst.com/learning/view/article/first-steps-in-imaris-image-analysis-software