Protocols

Safety

• Hazard assessment for Treseder Lab

• Precautions to take in using drying ovens

• Field safety

• Checklist for planning field trips

• Bear safety

Quantum dots

Microbial Cages

Ectomycorrhizal colonization

Staining roots for arbuscular mycorrhizal colonization

Hyphal Extractions

Mycorrhizal characteristics

PVLG slide mounting medium

Litter Chemistry (from Jenny Talbot)

• Chemical fractionation via Total Dietary Fibre Analysis
  Cellulose Analysis via Updegraff Method
  Lignin Analysis via Acetyl Bromide Method
  Hemicellulose Analysis via Updegraff Method
  Soluble Sugar Analysis via Phenol-Sulfuric Acid Method
  Gel state NMR Sample Prep
  Gel state HSQC Protocol
  8000D Spex SamplePrep Tissue grinding procedure

Glomalin

• Bradford protein assay for glomalin

• ELISA assay for glomalin

• Calculation examples for Bradford protein and ELISA

• Precipitation and dialysis of extracted glomalin

Microplate analyses of ammonium, nitrate, and inorganic P

KCl extractions of ammonium and nitrate

Resin P extractions

Resin bags for N and P availability

Use of the phase contrast microscope

Measuring root turnover in minirhizotron images

Soil organic matter content

Extraction of water stable aggregates

Ergosterol assay for fungal biomass

Microbalance use

Radiocarbon labeling of lignocellulose

Lignocellulose extractions

Generation of protein-tannin complexes

Data sheet templates

• Soil organic matter template

• Microbalance template

• N mineralization template

• Colonization and hyphal lengths of AM fungi template

• Water stable aggregates template