Publications – Flanagan Lab

Selected Publications

For full list of publications, see PubMed or Google scholar

Neural stem cell fate and related publications

Yale, A.R., E. Kim, B. Gutierrez, J.N. Hanamoto, J.L. Nourse, M. Salvatus, R.F. Hunt, E.S. Monuki, L.A. Flanagan. Regulation of neural stem cell differentiation and brain development by MGAT5-mediated N-glycosylation. Stem Cell Reports 18(6), 1340–1354, 2023.

Yale, A.R., J.L. Nourse, K.R. Lee, S.N. Ahmed, J. Arulmoli, A.Y.L. Jiang, L.P. McDonnell, G.A. Botten, A.P. Lee, E.S. Monuki, M. Demetriou, L.A. Flanagan. Cell surface N-glycans influence electrophysiological properties and fate potential of neural stem cells. Stem Cell Reports 11(4): 869-82, 2018.

Featured Publication, Neural Cell News, 12.36, September 12, 2018

Featured in The Stem Cell Podcast, Ep. 125, September 11, 2018

Nourse, J.L., J.L. Prieto, A.R. Dickson, J. Lu, M.M. Pathak, F. Tombola, M. Demetriou, A.P. Lee, L.A. Flanagan. Membrane biophysics define neuron and astrocyte progenitors in the neural lineage. Stem Cells 32(3): 706-16, 2014.

Featured Publication, Neural Cell News, 7.37, September 18, 2013

Labeed, F.H.*, J. Lu*, H.J. Mulhall, S.A. Marchenko, K.F. Hoettges, L.C. Estrada, A.P. Lee, M.P. Hughes, L.A. Flanagan. Biophysical characteristics reveal neural stem cell differentiation potential. PLoS ONE 6(9):e25458, 2011.

*These two authors contributed equally to this work.

In top 10% most cited articles in PLoS ONE as of June 2017.

Flanagan, L.A.*, J. Lu*, L. Wang, S.A. Marchenko, N. Jeon, A.P. Lee, E.S. Monuki. Unique dielectric properties distinguish stem cells and their differentiated progeny. Stem Cells 26(3): 656-665, 2008.

*These two authors contributed equally to this work.

Featured Publication: Biology News Net, Southern California Biomedical Council, and Cell Therapy News

Mangale, V.S., K.E. Hirokawa, P.R.V. Satyaki, N. Gokulchandran, S. Chickbire, L. Subramanian, A.S. Shetty, B. Martynoga, J. Paul, M.V. Mai, Y. Li, L.A. Flanagan, S. Tole, E.S. Monuki. Lhx2 selector activity specifies cortical identity and suppresses hippocampal organizer fate. Science 319: 304-309, 2008.

Human neural stem cells and their interactions in the niche and related publications

Arulmoli, J., H.J. Wright, D.T.T. Phan, U. Sheth, R.A. Que, G.A. Botten, M. Keating, E.L. Botvinick, M.M. Pathak, T.I. Zarembinski, D.S. Yanni, O.V. Razorenova, C.C.W. Hughes, L.A. Flanagan. Combination scaffolds of salmon fibrin, hyaluronic acid, and laminin for human neural stem cell and vascular tissue engineering. Acta Biomaterialia 43: 122-138, 2016.

Que, R.A.*, J. Arulmoli*, N.A. Da Silva, L.A. Flanagan#, S-W. Wang#. Recombinant collagen scaffolds as substrates for human neural stem/progenitor cells. Journal of Biomedical Materials Research: Part A 106(5): 1363-72, 2018.

*These two authors contributed equally to this work; #Co-corresponding authors.

Arulmoli, J., M.M. Pathak, L.P. McDonnell, J.L. Nourse, F. Tombola, J.C. Earthman, L.A. Flanagan. Static stretch affects neural stem cell differentiation in an extracellular matrix-dependent manner. Scientific Reports 5: 8499, 2015.

Featured Publication, Neural Cell News, 9.06, February 18, 2015

Sharp, K.G., A.R. Dickson, S.A. Marchenko, K.M. Yee, P.N. Emery, I. Laidmäe, R. Uibo, E.S. Sawyer, O. Steward, L.A. Flanagan. Salmon fibrin treatment of spinal cord injury promotes functional recovery and density of serotonergic innervation. Experimental Neurology 235(1): 345-56, 2012.

Huang, C.P., J. Lu, H. Seon, A.P. Lee, L.A. Flanagan, H-Y. Kim, A.J. Putnam, N. Jeon. Engineering microscale cellular niches for three-dimensional multicellular co-cultures. Lab on a Chip 9(12): 1740-1748, 2009.

Ju, Y., P.A. Janmey, M.E. McCormick, E.S. Sawyer, L.A. Flanagan. Enhanced neurite growth from mammalian neurons in three-dimensional salmon fibrin gels. Biomaterials 28: 2097-2108, 2007.

Flanagan, L.A., L.M. Rebaza, S. Derzic, P.H. Schwartz, E.S. Monuki. Regulation of human neural precursor cells by laminin and integrins. J. Neurosci. Res. 83(5): 845-856, 2006.

Chung, B.*, L.A. Flanagan*, S. Rhee, P.H. Schwartz, A.P. Lee, E.S. Monuki, N. Jeon. Human neural stem cell growth and differentiation in a gradient-generating microfluidic device. Lab on a Chip 5(4): 401-406, 2005. *These two authors contributed equally to this work.

Yeung, T., P.C. Georges, L.A. Flanagan, B. Marg, M. Ortiz, M. Funaki, N. Zahir, W. Ming, V. Weaver, P.A. Janmey. Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion. Cell Motility and the Cytoskeleton 60(1): 24-34, 2005.

Flanagan, L.A., Y. Ju, B. Marg, M. Osterfield, P.A. Janmey. Neurite branching on deformable substrates. Neuroreport 13(18): 2411-5, 2002.

Label free technologies to identify cell phenotype and enrich distinct cell populations and related publications

Adams, T.N.G., A.Y.L. Jiang, N.S. Mendoza, C.C. Ro, D.-H. Lee, A.P. Lee, L.A. Flanagan. Label-free enrichment of fate-biased human neural stem and progenitor cells. Biosensors and Bioelectronics 152: 111982, 2020.

Jiang, A.Y.L., A.R. Yale, M. Aghaamoo, D.-H. Lee, A.P. Lee, T.N.G. Adams, L.A. Flanagan. High-throughput continuous dielectrophoretic separation of neural stem cells. Biomicrofluidics 13(6): 064111, 2019. (Invited submission)

Liu, Y, A.Y.L. Jiang, E. Kim, C.C. Ro, T.N.G. Adams, L.A. Flanagan, T.J. Taylor, M.A. Hayes. Identification of neural stem and progenitor cell subpopulations using DC insulator-based dielectrophoresis. Analyst 144: 4066-72, 2019.

Simon, M.G., Y. Li, J. Arulmoli, L.P. McDonnell, A. Akil, J.L. Nourse, A.P. Lee, L.A. Flanagan. Increasing label-free stem cell sorting capacity to reach transplantation-scale throughput. Biomicrofluidics 8(6): 064106, 2014.

Featured Publication, Neural Cell News, 7.37, September 18, 2013

Lu, J., C.C. Barrios, A.R. Dickson, J.L. Nourse, A.P. Lee, L.A. Flanagan. Advancing practical usage of microtechnology: a study of the functional consequences of dielectrophoresis on neural stem cells. Integrative Biology 4(10): 1223-36, 2012.

Prieto, J.L., J. Lu, J.L. Nourse, L.A. Flanagan, A.P. Lee. Frequency discretization in dielectrophoretic assisted cell sorting arrays to isolate neural cells. Lab on a Chip 12(12): 2182-2189, 2012.

*These two authors contributed equally to this work.

In top 10% most cited articles in PLoS ONE as of June 2017.

Wang, L., J. Lu, S.A. Marchenko, E.S. Monuki, L.A. Flanagan, A.P. Lee. Dual frequency dielectrophoresis with interdigitated sidewall electrodes for microfluidic flow-through separation of beads and cells. Electrophoresis 30(5): 782-791, 2009.

*These two authors contributed equally to this work.

Featured Publication: Biology News Net, Southern California Biomedical Council, and Cell Therapy News

Wang, L., L.A. Flanagan, N. Jeon, E.S. Monuki, A.P. Lee. Dielectrophoresis switching with vertical sidewall electrodes for microfluidic flow cytometry. Lab on a Chip 7(9): 1114-1120, 2007.

Wang, L., L.A. Flanagan, A.P. Lee. Side-wall vertical electrodes for lateral field microfluidic applications. J. Microelectromechanical Systems 16(2): 454-461, 2007.

Selected other publications

Ellefsen, K.L^., A. Chang, J.R. Holt, J.L. Nourse, J. Arulmoli, A. Mekhdjian, H. Abuwarda, F. Tombola, L.A. Flanagan, A.R. Dunn, I. Parker, M.M. Pathak. Myosin-II mediated traction forces evoke localized Piezo1 Ca²⁺flickers. Communications Biology 2: 298, 2019.

Pathak, M.M., J.L. Nourse, T. Tran, J. Hwe, J. Arulmoli, D.T. Le, E. Bernardis, L.A. Flanagan, F. Tombola. Stretch-activated ion channel Piezo1 directs lineage choice in human neural stem cells. PNAS 111(45): 16148-16153, 2014.

Kautz, R., L. Phan, J. Arulmoli, J.P. Kerr, M. Naeim, J. Long, A. Allevato, J.E. Leal-Cruz, L. Le, P. Derakhsha, F. Tombola, L.A. Flanagan, A.A. Gorodetsky. Growth and spatial control of murine neural stem cells on reflectin films. ACS Biomaterials, 6: 1311-20, 2020.

Phan, L., R. Kautz, J. Arulmoli, I.H. Kim, D.T. Le, M.A. Shenk, M.M. Pathak, L.A. Flanagan#, F. Tombola#, A.A. Gorodetsky#. Reflectin as a material for neural stem cell growth. Applied Materials & Interfaces 8 (1): 278-84, 2016.

#Co-corresponding authors.

Hooshmand M.J., H.X. Nguyen, K.M. Piltti, F. Benavente, S. Hong, L.A. Flanagan, N. Uchida, B.J. Cummings, A.J. Anderson. Neutrophils induce astroglial differentiation and migration of human neural stem cells via C1q and C3a synthesis. J. Immunol 199(3): 1069-85, 2017.

Piltti K.M., G.M. Funes, S.N. Avakian, A.A. Salibian, K.I. Huang, K. Carta, N. Kamei, L.A. Flanagan, E.S. Monuki, N. Uchida, B.J. Cummings, A.J. Anderson. Increasing human neural stem cell transplantation dose alters oligodendroglial and neuronal differentiation after spinal cord injury. Stem Cell Reports 8(6): 1534-48, 2017.

Flanagan, L.A., J. Chou, H. Falet, R. Neujahr, J.H. Hartwig, T.P. Stossel. Filamin A, the Arp2/3 complex, and the morphology and function of cortical actin filaments in human melanoma cells. J. Cell Biol. 155(4): 511-8, 2001.

Featured in Journal of Cell Biology “In This Issue”, 155(4): 494, “Battle of the actin junctions”

Gleeson, J.G., P.T. Lin, L.A. Flanagan, C.A. Walsh. Doublecortin is a microtubule-associated protein and is expressed widely by migrating neurons. Neuron 23: 257-271, 1999.

Shah, J.V., L.A. Flanagan, P.A. Janmey, J.-F. Leterrier. Bidirectional translocation of neurofilaments along microtubules mediated in part by dynein/dynactin. Mol. Biol. Cell 11(10): 3495-3508, 2000.

Shah, J.V., L.A. Flanagan, D. Bahk, P.A. Janmey. Reptation of microtubules in F-actin networks: effects of filament stiffness and network topology on reptation dynamics. Material properties of the cell. Proc. of the 1997 Mat. Res. Soc. 489: 27-31, 1998.