Publications

  1. Liang EI, Mah EJ, Yee AF, Digman MA. Correlation of focal adhesion assembly and disassembly with cell migration on nanotopography.
    Integr Biol (Camb). 2017; 9(2): 145-155.
  2. de Vega GB, Ceballos JA, Anzalone A, Digman MA, Gratton E.
    A laser-scanning confocal microscopy study of carrageenan in red algae from seaweed farms near the Caribbean entrance of the Panama Canal.
    J Appl Phycol. 2017; 29(1): 495-508.
  3. Romero-López M, Trinh AL, Sobrino A, Hatch MMS, Keating MT, Fimbres C, Lewis DE, Gershon PD, Botvinick EL, Digman MA, Lowengrub JS, Hughes CCW.
    Recapitulating the human tumor microenvironment: Colon tumor-derived extracellular matrix promotes angiogenesis and tumor cell growth.
    Biomaterials. 2017; 116: 118-129. PMCID: PMC5226635
  4. Sameni S., Syed, A., Marsh, J.L, and Digman, M.A. The phasor-FLIM fingerprints reveal shifts from OXPHOS to enhanced glycolysis in Huntington Disease. Sci Rep. 2016 Oct 7;6:34755. doi: 10.1038/srep34755. 
  5. Rachel Cinco, Michelle A Digman, Enrico Gratton, and Ulrike Luderer.
    Spatial characterization of bioenergetics and metabolism of primordial to preovulatory follicles in whole ex vivo murine ovary. Biol Reprod. 2016; [Epub ahead of print].
  6. Luca Digiacomo, Michelle A Digman, Enrico Gratton, and Giulio Caracciolo.
    Development of an image Mean Square Displacement (iMSD)-based method as a novel approach to study the intracellular trafficking of nanoparticles.
    Acta Biomater. 2016; 42: 189-198.
  7. Hongtao Chen, Enrico Gratton and Michelle A Digman. “Self-assisted optothermal trapping of gold nanorods under two-photon excitation”. Methods and Applications in Fluorescence, Volume 4, Number 3.
  8. Ning Ma, Michelle A Digman, Leonel Malacrida, and Enrico Gratton.
    Measurements of absolute concentrations of NADH in cells using the phasor FLIM method.  Biomed Opt Express. 2016; 7(7): 2441-2452. PMCID: PMC4948605
  9. Digman,M.A. “3D Nanoimaging and Detection of Molecular Flow using the nSPIRO Method,” in Optics in the Life Sciences, OSA Technical Digest (online) (Optical Society of America, 2015), paper NM4C.1. https://www.osapublishing.org/abstract.cfm?URI=NTM-2015-NM4C.1
  10. Olivera-Couto, A., Salzman, V., Mailhos, M., Digman, M.A., Gratton, E., and Aguilar, P.S.. Eisosomes are dynamic plasma membrane domains showing Pil1-Lsp1 heteroligomer binding equilibrium. Biophys J. 2015; 108(7): 1633-1644.
  11. Moens, J.P., Digman, M.A. and Gratton, E.. Modes of diffusion of Cholera toxin bound to GM1 on live cell membrane by image mean square displacement analysis. Biophys J. 2015; 108(6): 1448-1458.
  12. Chen, H., Gratton, E.,and Digman, M.A. Spectral properties and dynamics of gold nanorods revealed by EMCCD-based spectral phasor method. Microsc Res Tech. 2015; 78(4): 283-293.
  13. Kang,D., Monsur M., Zhang, K., Huang, S.S., Peterson, E., Digman, M.A., Gratton, E., and Zhao, W. Rapid detection of single bacteria in unprocessed blood using Integrated Comprehensive Droplet Digital Detection. Nat Commun. 2014; 5: 5427. PMCID: PMC4243214
  14. Adu-Gyamf, E.i, Soni, S.P., Jee, C.L., Digman, M.A., Gratton, E., and Stahelin, R.V.A loop region in the N-terminal domain of Ebola virus VP40 is important in viral assembly, budding, and egress. Viruses. 2014; 6(10): 3837-3854. PMCID: PMC4213565
  15. Pate, K.T., Stringari, C., Sprowl Tanio, S., Wang, K, TeSlaa, T., Hoverter,N.P., McQuade, M., Garne, C., Digman, M.A., Teitell, M.A., Edwards, R.A., Gratton, E., Waterman, M.L. “Wnt Signaling Directs a Metabolic Program of Glycolysis and Angiogenesis in Colon Cancer,” The EMBO Journal; May 2014,33(13)1454-1473;DOI: 10.15252/embj.201488598
  16. Chiu, C., Aguilar, J.S, Tsai, C.Y., Wu, G., E. Gratton and Digman, M.A. ”Nanoimaging of focal adhesions in 3D”, PLOSONE, Published: June 24, 2014 DOI: 10.1371/journal.pone.0099896
  17. Sharma, H., Digman, M.A., Felsinger, N., Gratton, E. and Khine, M.. “Enhanced emission of fluorophores on shrink-induced wrinkled composite structures.” Optical Materials Express. 2014; 4(4): 753-763.
  18. Pozzi,D., Marchini, C., Cardarelli,F., Salomone, F., Coppola, S., Montani, M., Zabaleta, M.E., Digman, M.A., Gratton, E., Colapicchioni, V., and Caracciolo, G.. “Mechanistic evaluation of the transfection barriers involved in lipid-mediated gene delivery: Interplay between nanostructure and composition.” Biochim Biophys Acta. 2014; 1838(3): 957-967. PMCID: PMC3940149
  19. James, N.G., Digman, M.A., Ross, J.A., Barylko, B., Wang, L., Li,J., Chen, Y., Mueller, J.D., Gratton, E., Albanesi, J.P.,, and Jameson, D.M. “A mutation associated with centronuclear myopathy enhances the size and stability of dynamin 2 complexes in cells.” Biochim Biophys Acta. 2014; 1840(1): 315-321. PMCID: PMC3859711
  20. Chiu, C., Digman, M.A., and Gratton, E., “Cell matrix remodeling ability shown by image spatial correlation”. J Biophys. 2013; 2013, 532030. PMCID: PMC3725897
  21. Chiu, C., Digman, M.A. and Gratton, E. “Measuring Actin Flow in 3D Cell Protrusion.” Biophys J. 2013; 105(8): 1746-1755. PMCID: PMC3797595
  22. Hinde, E. , Digman, M.A., Hahn, K.M., and Gratton, E.”Millisecond spatiotemporal dynamics of FRET biosensors by the pair correlation function and the phasor approach to FLIM.” Proc Natl Acad Sci USA. 2013; 110(1): 135-140. PMCID: PMC3538204
  23. Adu-Gyamfi, E, Soni, S.P., Xue, Y., Digman, M.A., Gratton, E. and Stahelin RV.” The Plasma Membrane Induces the Membrane Penetration of the Ebola Virus Matrix Protein: A Key Step in VP40 Oligomerization and Viral Egress.” J Biol Chem. 2013; 288(8): 5779-5789. PMCID: PMC3581432
  24. Coppola, S., Pozzi, D., Candeloro De Sanctis, S., Digman, M.A., Gratton, E. and Caracciolo, G. “Quantitative measurement of intracellular transport of nanocarriers by spatio-temporal image correlation spectroscopy.” Methods Appl Fluoresc. 2013; 1(1): 015005.
  25. Andrews, L.M., Jones, M.R., Digman, M.A., and Gratton, E. “Detecting Pyronin Y labeled RNA transcripts in live cell microenvironments by phasor-FLIM analysis.” Methods Appl Fluoresc. 2013; 1(1): 015001
  26. Torno, K., Wright, B.K., Jones, M.R., Digman, M.A., Gratton, E., and Phillips, M. “Real-time analysis of metabolic activity within lactobacillus acidophilus by phasor fluorescence lifetime imaging microscopy of NADH.” Curr Microbiol. 2013; 66(4): 365-367.
  27. Adu-Gyamfi E, Digman, M.A., Gratton E, Stahelin RV. “Single-Particle Tracking Demonstrates that Actin Coordinates the Movement of the Ebola Virus Matrix Protein. Biophys J. 2012 Nov 7;103(9):L41-3.
  28. Wright BK, Andrews LM, Jones MR, Stringari C, Digman M.A., Gratton E. “Phasor-flim analysis of NADH distribution and localization in the nucleus of live progenitor myoblast cells.” Microsc Res Tech. 2012 Dec;75(12):1717-22.
  29. Whiteside, M.D., Digman, M.A., Grattton, E. and Treseder, K.K. Organic Nitrogen Uptake By Arbuscular Mycorrhizal. Soil Biology & Biochemistry Volume 55, December 2012, Pages 7-13.
  30. Coppola, S., Estrada, L.C., Digman, MA., Pozzi, P., Cardarelli, F., Gratton, E., and Caracciolo, G. “Intracellular trafficking of cationic liposome–DNA complexes in living cells.” Soft Matter. 2012; 8(30): 3919-3927
  31. Wright, B.K., Andrews, L.M., Markham, J., Jones, M.R., Stringari, C., Digman, M.A., and Gratton, E. “NADH distribution in live progenitor stem cells by phasor-fluorescence lifetime image microscopy.” Biophys J. 2012; 103(1): L7-L9
  32. Adu-Gyamfi, E., Digman, M.A., Gratton, E., and Stahelin, R.V. “Investigation of Ebola VP40 assembly and oligomerization in live cells using number and brightness analysis.” Biophys J. 2012; 102(11): 2517-2525
  33. James, N.G., Digman, M.A., Gratton, E., Barylko, B., Ding, X., Albanesi, J.P., Goldberg, M.S. and Jameson, D.M. “Number and brightness analysis of LRRK2 oligomerization in live cells.” Biophys J. 2012; 102(11): L41-L43
  34. Battisti, A., Digman, M.A., Gratton, E., Storti, B., Beltram, F., and Bizzarri, R. Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging. Chem Commun (Camb). 2012; 48(42): 5127-5129.
  35. Digman, M.A. and Gratton, E.Scanning image correlation spectroscopy. 2012; 34(5): 377-385.
  36. Zhou, S., Lo, W.L., Suhalim, J.L., Digman, M.A., Gratton, E., Nie, Q., and Lander, A.D. Free extracellular diffusion creates the Dpp morphogen gradient of the Drosophila wing disc. Curr Biol. 2012; 22(8): 668-675.
  37. Kniazeva, E., Weidling, J.W., Singh, R., Botvinick, E.L., Digman, M.A., Gratton, E., and Putnam, A.J. Quantification of local matrix deformations and mechanical properties during capillary morphogenesis in 3D. Integr Biol (Camb). 2012; 4(4): 431-439.
  38. Hinde, E., Digman, M.A., Welch, C., Hahn, K.M. and Gratton, E.. Biosensor Förster resonance energy transfer detection by the phasor approach to fluorescence lifetime imaging microscopy. Microsc Res Tech. 2012; 75(3): 271-281.
  39. Hinde, E., Cardarelli, F., Digman, M.A., and Gratton, E.. Changes in chromatin compaction during the cell cycle revealed by micrometer-scale measurement of molecular flow in the nucleus. Biophys J. 2012; 102(3): 691-697.
  40. Stafford, R.L., Hinde, E., Knight, M.J., Pennella, A., Ear, J., Digman, M.A. Gratton, E., Bowie, J.U. “Tandem SAM Domain Structure of Human Caskin1: A Presynaptic, Self-Assembling Scaffold for CASK. “ Structure, 7 December 2011, Vol. 19, Issue 12, pp. 1826-1836.
  41. Hinde, E., Cardarelli, F., Digman, M.A., and Gratton, E., “The impact of mitotic versus interphase chromatin architecture on the molecular flow of EGFP by pair correlation analysis.” Biophys J. 2011; 100(7): 1829-1836.
  42. Stringari, C., Cinquin, A., Cinquin, O., Digman, M.A., Donovan, P., and Gratton, E., “Phasor approach to fluorescence lifetime microscopy distinguishes different metabolic states of germ cells in live tissue.” Natl. Acad. Sci. USA. 2011; 108(33): 13582-13587.
  43. Lanzano, L., Digman, M.A., Fwu, P., Giral H., Levi, M., and Gratton, E., “Nanometer-scale imaging of microvilli in opossum kidney cells by the modulation tracking method.” J Biophotonics 2011; 4(6): 415–424.
  44. Marchin, C.,Montani, M.,Alfonsi, C, Amici, A.,Digman, M.A., Sanchez, S., Gratton,E., Amenitsch, H.,Pozzi, D.,and Caracciolo, G., “Evidence of a novel mechanism in lipid-mediated gene delivery.” Cancer Gene Ther. 2011; 18(8): 543-552.
  45. Ross, J.A.,Digman, M.A., Wang, L., Gratton, E., Albanesi, J.P., and Jameson, D.M. “Oligomerization State of Dynamin 2 in Cell Membranes Using TIRF and Number and Brightness Analysis.” Biophys J. 2011; 100(3): L15-17.
  46. Choi, C.K., Zareno, J.,Digman, M.A.,Gratton, E., and Horwitz, A.R., “Cross-Correlated Fluctuation Analysis Reveals Phosphorylation-Regulated Paxillin-FAK Complexes in Nascent Adhesions” Biophys J. 2011; 100(3): 583-592.
  47. Vetri, V.,Ossato,G.,Militello, V.,Digman, M.A.,Leone, M., and Gratton, E., “Fluctuation methods to study protein aggregation in live cells: Concanavalin A oligomers formation” Biophys J; Biophys J. 2011; 100(3): 774-783.
  48. A Kim, H. Sanabria, M.A Digman, Enrico Gratton, P. Schwille, W. R. Zipfel, and M. N. Waxham. “Quantifying translational mobility in neurons: comparison between current optical techniques.” J Neurosci. 2010; 30(49): 16409-16416
  49. Fu, C., Ossato, G., Long, M., Digman, A., Gopinathan, A.,Lee, L.P., Gratton, E., Khine, M. “Bimetallic Nanopetals for Thousand-Fold Fluorescence Enhancements.” Applied Physics Letters; 97, 203101 (2010). Cover image.
  50. Hinde, E., Cardarelli, F., Digman, M.A., and Gratton, E., “In vivo pair correlation analysis of EGFP intranuclear diffusion reveals DNA-dependent molecular flow.” Natl. Acad. Sci. U S A. 2010 Sep 21;107(38):16560-5. Epub 2010 Sep 7. PMID: 20823232.
  51. Kuo, W.J., Digman, M.A., Lander, A.D.”Heparan Sulfate Acts as a BMP Co-receptor by Facilitating Ligand-induced Receptor Hetero-oligomerization.” Biol. Cell. 2010; 21(22): 4028-4041
  52. M Presman, L. D. Alvarez, V. Levi, Silvina E., M. A. Digman, M. A. Martí, A. S. Veleiro, G. Burton, and A. Pecci. “Insights on glucocorticoid receptor activity modulation through the binding of rigid steroids.” PLoS One. 2010; 5(10): e13279
  53. Rossow, M., Sasaki, J.M., Digman, M.A., and Gratton, E., “Raster image correlation spectroscopy in live cells.” Nature Protocols, V5, P1761–1774, doi:10.1038/nprot.2010.122.
  54. Ossato G., Digman M.A., Aiken C., Lukacsovich T., Marsh J.L., Gratton E. “A two step path to inclusion formation of Huntingtin peptides revealed by N&B analysis.” Biophys J. 2010 Jun 16;98(12):3078-85. PMID: 20550921.
  55. Yoon, Y., Tong, J., Lee, P.J., Albanese, A., Bhardwaj, N., Källberg. M., Digman, M.A., Lu, H., Gratton, E., Shin, Y., Cho, W. “Molecular basis of the potent membrane remodeling activity of the epsin1 ENTH domain.” Biol. Chem. 2010; 285(1): 531-540. PMCID: PMC2804201.
  56. Digman, M.A. and Gratton E. “Imaging barriers to diffusion by pair correlation functions.” Biophys J. 2009; 97(2): 665-673. PMCID: PMC2711318.
  57. Digman, M.A.,P.W., Choi, C., Horwitz, A.R., Gratton, E. “Mapping the stoichiometry of molecular complexes at focal adhesions in live cells.” Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2170-5. PMCID: 2630200.
  58. Digman, M.A., P.W., Horwitz, A.R., Gratton, E. “Mapping protein complexes in living cells from laser scanning confocal image sequences: the ccRICS method.” Biophys J. 2009 Jan;96(2): 707-16.
  59. A. Digman and E. Gratton.”Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy, applications in systems biology.” WIREs Syst Biol Med. 2009; 1(2): 273-282.
  60. Caracciolo, G., Caminiti, R., Digman, M.A., Gratton, E., Sanchez, S. “Efficient escape from endosomes determines the superior efficiency of multicomponent lipoplexes,”J Phys Chem B. 2009 Apr 16;113(15):4995-7.
  61. Vetri V., Ossato G., Militello V., Digman, M.A., Leone M., and Gratton E. “Oligomerization of Concanavalin A in live cells detected by fluctuation analysis.” Eur Biophys J. 2009; 38(Suppl 1): S137, P-393.
  62. Gielen E, Smisdom N, Vandeven M, De Clercq B, Gratton E, Digman M., Rigo JM, Hofkens J, Engelborghs Y, Ameloot M. “Measuring Diffusion of Lipid-like Probes in Artificial and Natural Membranes by Raster Image Correlation Spectroscopy (RICS): Use of a Commercial Laser-Scanning Microscope with Analog Detection.” Langmuir. 2009 May 5;25(9): 5209-18.
  63. Digman, M.A., and Gratton, E. “Analysis of diffusion and binding in cells using the RICS approach.” Microscopy Res. Tech. 2008; Dec 9;72(4): 323-332. PMID: 19067357.
  64. Watson NB, Nelson E, Digman M., Thornburg JA, Alphenaar BW, McGregor WG. “RAD18 and associated proteins are immobilized in nuclear foci in human cells entering S-phase with ultraviolet light-induced damage.” Mutat Res. 2008 15;648(1-2): 23-31.
  65. Sanabria, H., Digman, M.A., Gratton E., and Waxham M.N. “Spatial Diffusivity and Availability of Intracellular Calmodulin” Biophys J. 2008 Dec 15;95(12): 6002-15.
  66. M. Brown, Dalal, R.,Hebert, B., Digman, M.A., Gratton, E., Horwitz, A.F., “Raster Image Correlation Spectroscopy (RICS) for Measuring Fast Protein Dynamics and Concentrations with a Commercial Laser Scanning Confocal Microscope.” J Microsc. 2008 Jan;229(Pt 1): 78-91.
  67. Digman, M.A., R. Dalal, C.M. Brown, A.F. Horwitz, E. Gratton, “Mapping the number     of molecules and brightness in the laser scanning microscope.” Biophys J. 2008 Mar 15;94(6): 2320-32.
  68. Digman M.A., C.M. Brown, A. F. Horwitz, W.W. Mantulin, and E. Gratton. “Paxillin dynamics measured during adhesion assembly and disassembly by correlation spectroscopy.” Biophys J. Apr 1;94(7): 2819-31, 2008.
  69. Digman M.A., V. R. Caiolfa, M. Zamai, and E. Gratton. “The Phasor approach to fluorescence lifetime imaging analysis.” Biophys J. 2008 Jan 15;94(2): L14-6.
  70. Dalal, R.B., A. Digman, A. F. Horwitz, V. Vetri, and E. Gratton. “Determination of particle number and brightness using a laser scanning confocal microscope operating in the analog mode. Microsc. Res. Tech. 2008 Jan;71(1): 69-81.
  71. Caiolfa V.R., M. Zamai, G. Malengo, A. Andolfo, C. D. Madsen, J. D. B. Sutin, A. Digman, E. Gratton, F. Blasi, and N. Sidenius. “Monomer–dimer dynamics and distribution of GPI-anchored uPAR are determined by cell surface protein assemblies.” J Cell Biol 2007 179(5): 1067-1082.
  72. Gokhale NA, A. Abraham, A. Digman, E. Gratton, and W. Cho. “Phosphoinositide specificity of and mechanism of lipid domain formation by annexin A2-p11 heterotetramer.” J. Biol. Chem. 2005, 280(52): 42831-40.
  73. Digman M.A., C.M. Brown , P. Sengupta , P.W. Wiseman, A.R. Horwitz, and E. Gratton E. “Measuring fast dynamics in solutions and cells with a laser scanning microscope.” J. 2005 Aug;89(2): 1317-27.
  74. Digman M.A., P. Sengupta, P.W. Wiseman, C.M. Brown, A.R. Horwitz, and E. Gratton. “Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure.” J. 2005 May;88(5): L33-6.
  75. Stahelin R.V., A. Digman, M. Medkova, B. Ananthanarayanan, H.R. Melowic, J.D. Rafter, and W. Cho. “Diacylglycerol-induced membrane targeting and activation of protein kinase Cepsilon: mechanistic differences between protein kinases Cdelta and Cepsilon.” J Biol Chem. 2005 May 20;280(20): 19784-93.
  76. Barcellona M.L., S. Gammon, T. Hazlett, A. Digman, and E. Gratton. “Polarized fluorescence correlation spectroscopy of DNA-DAPI complexes.” Microsc. Res. Tech. 2004 Nov; 65(4-5): 205-17.
  77. Inoue M., A. Digman, M. Cheng, S.Y. Breusegem, N. Halaihel, V. Sorribas, W.W. Mantulin, E. Gratton, N.P. Barry, and M. Levi. “Partitioning of NaPi cotransporter in cholesterol-, sphingomyelin-, and glycosphingolipid-enriched membrane domains modulates NaPi protein diffusion, clustering, and activity.” J. Biol. Chem. 2004 Nov 19;279(47): 49160-71.
  78. Stahelin R.V., A. Digman, M. Medkova, B. Ananthanarayanan, J.D. Rafter, H. Melowic, and W. Cho. “Mechanism of diacylglycerol-induced membrane targeting and activation of proteinkinase Cdelta.” J. Biol. Chem. 2004 Jul 9;279(28): 29501-12.
  79. Ananthanarayanan B., R.V. Stahelin , A. Digman, W. Cho, “Activation mechanisms of conventional protein kinase C isoforms are determined by the ligand affinity and conformational flexibility of their C1 domains.” J. Biol. Chem. 2003 Nov 21;278(47): 46886-94.
  80. Narducci, A.A., Yang, Y., Digman, M.A., Sipes, A.B., Ibers, J.A., “An investigation of the rare-earth telluride system BaLn2Te4 (Ln=Sm–Tm, Y): syntheses, crystal structures, and magnetic properties. Journal of Alloys and Compounds, 2000 May 24, 303-304: 432-439.