Publications

J115    Perinbam K, Kannan JVCA, Digman MA, Siryaporn A. A shift in central metabolism accompanies virulence activation in Pseudomonas aeruginosa. mBio. 2020; 11(2), e02730-18.

J114    Davis RT, Blake K, Ma D, Gabra MBI, Hernandez GA, Phung AT, Yang Y, Maurer D, Lefebvre AEYT, Alshetaiwi H, Xiao Z, Liu J, Locasale JW, Digman MA, Mjolsness E, Kong M, Werb Z, Lawson DA. Transcriptional diversity and bioenergetic shift in human breast cancer metastasis revealed by single-cell RNA sequencing. Nat Cell Biol. 2020; 22(3): 310-320.

J113    Sameni S, Zhang R, Digman MA. Number and molecular brightness analysis reveals Htt25Q protein aggregation upon the uptake of Htt97Q aggregates.
Biochem Biophys Res Commun. 2020; 522(1): 133-137.

2019

J112    Trivedi P., Palomba F., Niedzialkowska E., Digman M.A., Gratton E., Stukenberg P.T. The inner centromere is a biomolecular condensate scaffolded by the chromosomal passenger complex. Nat. Cell Biol. 2019;21:1127–1137. doi: 10.1038/s41556-019-0376-4.

J111    Murata MM, Kong X, Moncada E, Chen Y, Imamura H, Wang P, Berns MW, Yokomori K, Digman MA. NAD+ consumption by PARP1 in response to DNA damage triggers metabolic shift critical for damaged cell survival. Mol Biol Cell. 2019; 30(20): 2584-2597. PMCID: PMC6740200

J110    Ma N, de Mochel NR, Pham PD, Yoo TY, Cho KWY, Digman MA. Label-free assessment of pre-implantation embryo quality by the Fluorescence Lifetime Imaging Microscopy (FLIM)-phasor approach. Sci Rep. 2019; 9(1), 13206. PMCID: PMC6744410

J109    Trivedi P, Palomba F, Niedzialkowska E, Digman MA, Gratton E, Stukenberg PT. The inner centromere is a biomolecular condensate scaffolded by the chromosomal passenger complex. Nat Cell Biol. 2019; 21(9): 1127-1137.

J108    Dong Y, Sameni S, Digman MA, Brewer GJ. Reversibility of age-related oxidized free NADH redox states in Alzheimer’s disease neurons by imposed external Cys/CySS redox shifts. Sci Rep. 2019; 9(1), 11274. PMCID: PMC6677822

J107    Chen H, Ma N, Kagawa K, Kawahito S, Digman M, Gratton E. Widefield multifrequency fluorescence lifetime imaging using a two-tap complementary metal-oxide semiconductor camera with lateral electric field charge modulators. J.Biophotonics. 2019; 12(5), e201800223.

J106    Planes N, Digman MA, Vanderheyden PPML, Gratton E, Caballero-George C. Number and brightness analysis to study spatio-temporal distribution of the angiotensin II AT1 and the endothelin-1 ETA receptors: Influence of ligand binding. Biochim Biophys Acta Gen Subj. 2019; 1863(5): 917-924.

J105    Dong Y, Digman MA, Brewer GJ. Age- and AD-related redox state of NADH in subcellular compartments by fluorescence lifetime imaging microscopy. Geroscience 2019; 41(1): 51-67. PMCID: PMC6423217

2018

J104    Ma N, Kamalakshakurup G, Aghaamoo M, Lee AP, Digman MA. Label-Free Metabolic Classification of Single Cells in Droplets Using the Phasor Approach to Fluorescence Lifetime Imaging Microscopy. Cytometry A. 2018 Dec 11. doi: 10.1002/cyto.a.23673. [Epub ahead of print] PMID:30536717

J103.Mah EJ, Lefebvre AEYT, McGahey GE, Yee AF, Digman MA. Collagen density modulates triple-negative breast cancer cell metabolism through adhesion-mediated contractility. Sci Rep. 2018 Nov 20;8(1):17094. doi: 10.1038/s41598-018-35381-9.

J102.   Chen H, Ma N, Kagawa K, Kawahito S, Digman M, Gratton E. Widefield multi-frequency fluorescence lifetime imaging using a two-tap CMOS camera with lateral electric field charge modulators. J Biophotonics. 2018 Nov 12:e201800223. doi: 10.1002/jbio.201800223. [Epub ahead of print] PMID:30421535

J101.   Anzalone A, Chacko JV, Nishi RA, Dumont C, Smith D, Shea LD, Digman MA, Cummings BJ, Anderson AJ. Feasibility study on mouse live imaging after spinal cord injury and poly(lactide-co-glycolide) bridge implantation. J Biomed Opt. 2018 Jun;23(6):1-6. doi: 10.1117/1.JBO.23.6.065007

J100.   Kim SM, Nguyen TT, Ravi A, Kubiniok P, Finicle BT, Jayashankar V, Malacrida L, Hou J, Robertson J, Gao D, Chernoff J, Digman MA, Potma EO, Tromberg BJ, Thibault P, Edinger AL. PTEN deficiency and AMPK activation promote nutrient scavenging and anabolism in prostate cancer cells. Cancer Discov. 2018; 8(7): 866-883. PMCID: PMC6030497

J99.     Mäntylä E, Chacko JV, Aho V, Parrish CR, Shahin V, Kann M, Digman MA, Gratton E, Vihinen-Ranta M.  Viral highway to nucleus exposed by image correlation analyses.

Sci Rep. 2018 Jan 18;8(1):1152. doi: 10.1038/s41598-018-19582-w.

J98.     Lee DH, Li X, Ma N, Digman MA, Lee AP. Rapid and label-free identification of single leukemia cells from blood in a high-density microfluidic trapping array by fluorescence lifetime imaging microscopy. Lab Chip. 2018 May 1;18(9):1349-1358. doi: 10.1039/c7lc01301a.

J97.     Kong, L., Murata, M. M. and Digman, M. A. Absence of REV3L promotes p53-regulated cancer cell metabolism in cisplatin-treated lung carcinoma cells. Biochem Biophys Res Commun 496, 199-204, doi:10.1016/j.bbrc.2018.01.026 (2018).

J96.     Sameni, S., Malacrida, L., Tan, Z.and Digman, M. A. Alteration in Fluidity of Cell Plasma Membrane in Huntington Disease Revealed by Spectral Phasor Analysis. Scientific Reports 8, 734, doi:10.1038/s41598-018-19160-0 (2018).

2017

J95.     Trinh, A., Chen,H., Chen, y., Hu, Y. Li, Z., Siegel, E.R., Linskey, M.E., Wang, P.W., Digman*, M.A. and Zhou*, Y., “Tracking Functional Tumor Cell Subpopulations of Malignant Glioma by Phasor Fluorescence Lifetime Imaging Microscopy of NADH”, Y.  Cancers 2017, 9(12), 168; doi:10.3390/cancers9120168 *co-corresponding authors

J94.       Davey RJ, Digman MA, Gratton E, Moens PDJ. Quantitative image mean squared displacement (iMSD) analysis of the dynamics of profilin 1 at the membrane of live cells. Methods. 2018 May 1;140-141:119-125. doi: 10.1016/j.ymeth.2017.12.002. Epub 2017 Dec 11.PMID:29242135

J95.     Digiacomo L, Cardarelli F, Pozzi D, Palchetti S, Digman MA, Gratton E, Capriotti AL, Mahmoudi M, Caracciolo G. An apolipoprotein-enriched biomolecular corona switches the cellular uptake mechanism and trafficking pathway of lipid nanoparticles. Nanoscale. 2017; 9(44): 17254-17262. PMCID: PMC5700750

J96.     Liu L, Zhang SX, Liao W, Farhoodi HP, Wong CW, Chen CC, Ségaliny AI, Chacko JV, Nguyen LP, Lu M, Polovin G, Pone EJ, Downing TL, Lawson DA, Digman MA, Zhao W. Mechanoresponsive stem cells to target cancer metastases through biophysical cues. Sci Transl Med. 2017; 9(400), aan2966.

J93.     Herrington KA, Trinh AL, Dang C, O’Shaughnessy E, Hahn KM, Gratton E, Digman MA, Sütterlin C. Spatial analysis of Cdc42 activity reveals a role for plasma membrane-associated Cdc42 in centrosome regulation.  Mol Biol Cell. 2017 Jul 15;28(15):2135-2145. doi: 10.1091/mbc.E16-09-0665. Epub 2017 May 24.PMID:28539409.

J92.    Liang EI, Mah EJ, Yee AF, Digman MA. Correlation of focal adhesion assembly and disassembly with cell migration on nanotopography.    Integr Biol (Camb). 2017; 9(2): 145-155. PMCID: PMC5399776

J91.     de Vega GB, Ceballos JA, Anzalone A, Digman MA, Gratton E. A laser-scanning confocal microscopy study of carrageenan in red algae from seaweed farms near the Caribbean entrance of the Panama Canal.    J Appl Phycol. 2017; 29(1): 495-508.

J90.     Romero-López M, Trinh AL, Sobrino A, Hatch MMS, Keating MT, Fimbres C, Lewis DE, Gershon PD, Botvinick EL, Digman MA, Lowengrub JS, Hughes CCW.    Recapitulating the human tumor microenvironment: Colon tumor-derived extracellular matrix promotes angiogenesis and tumor cell growth.    Biomaterials. 2017; 116: 118-129. PMCID: PMC5226635

2016

J89.     Qiu, Y., Lin, C., Hinkle, P., Plett, T.S., Yang, C., Varghese Chacko, J. Digman, M.A., Yeh, L., Hsu, J. and Siwy, Z., Highly Charged Particles Cause a Larger Current Blockage in Micropores Compared to Neutral Particles. , American Chemical Society, ACS Nao 2016; 10: 8413-8422. doi: 10.1021/acsnano.6b03280

J88.     Sameni, A., Syed, A., Marsh, J.L., and Digman, M.A., The phasor-FLIM fingerprints reveal shifts from OXPHOS to enhanced glycolysis in Huntington Disease. Scientific Reports 6, Article number: 34755 (2016); doi:10.1038/srep34755.

J87.     Cinco,R., Digman, M.A., Gratton, E. and Luderer, U. Spatial characterization of bioenergetics and metabolism of primordial to preovulatory follicles in whole ex vivo murine ovary. Biol Reprod. 95(6):129. doi: 10.1095/biolreprod.116.142141. Epub 2016 Sep 28.

J86.     Digiacomo, L.,  Digman, M.A., Gratton, E., and Caracciolo, G. Development of an image Mean Square Displacement (iMSD)-based method as a novel approach to study the intracellular trafficking of nanoparticles. Acta Biomater. 2016; 42: 189-198.

J85.     Batista de Vega, G., Ceballos, J.A., Anzalone, A., Digman, M.A. and Gratton, E. A laser-scanning confocal microscopy study of carrageenan in red algae from seaweed farms near the Caribbean entrance of the Panama Canal. J Appl Phycol (2017) 29: 495. https://doi.org/10.1007/s10811-016-0914-4.

J84.     Chen, H., Gratton, E. and Digman, M.A. Self-assisted optothermal trapping of gold nanorods under two-photon excitation, Methods Appl Fluoresc. 2016; 4(3): 035003. PMCID: PMC5497913

J83.     Ma, N., Digman, M.A., Malacrida,L. and Gratton, E. Measurements of absolute concentrations of NADH in cells using the phasor FLIM method. Biomed. Opt. Express 7(7), 2441-2452 (2016)

J82.     Sosnik, J., Zheng, L., Rackauckas, C.V., Digman, M.A., Gratton, E., Nie, Q., and Schilling, T. Noise modulation in retinoic acid signaling sharpens segmental boundaries of gene expression in the embryonic zebrafish hindbrain. ELife. 2016; 5: e14034. PMCID: PMC4829421

2015

J81.     Hendrix, J., Baumgärtel, V., Schrimpf,  W., Ivanchenko, S., Digman, M.A., Gratton, E., Kräusslich, H., Müller, B., and Lamb, D., Live-cell observation of cytosolic HIV-1 assembly onset reveals RNA-interacting Gag oligomers. J. Cell Biol. 2015; 210(4): 629-626. doi: 10.1083/jcb.201504006.

J80.     Mieruszynski, S., Digman, M.A., Gratton, E., and Jones, M.R., Characterization of exogenous DNA mobility in live cells through fluctuation correlation spectroscopy. Scientific Reports 5, Article 13848 (2015); doi: 10.1038/srep13848

J79.     Kaixiang Zhang, Dong-Ku Kang, M Monsur Ali, Linan Liu, Louai Labanieh, Mengrou Lu, Hamidreza Riazifar, Thi N Nguyen, Jason A Zell, Michelle A Digman, Enrico Gratton, Jinghong Li, and Weian Zhao. Digital quantification of miRNA directly in plasma using integrated comprehensive droplet digital detection. Lab Chip. 2015; 15(21): 4217-4226. PMCID: PMC4631652

J78.     Z Tan, W Dai, T G M van Erp, J Overman, A Demuro, Michelle A Digman, A Hatami, R Albay, E M Sontag, K T Potkin, S Ling, F Macciardi, W E Bunney, J D Long, J S Paulsen, J M Ringman, I Parker, C Glabe, L M Thompson, W Chiu, and S G Potkin. Huntington’s disease cerebrospinal fluid seeds aggregation of mutant huntingtin.Mol Psychiatry. 2015; 20(11): 1286-1293.

J77.     Adu-Gyamfi, E., Johnson, K.A., Fraser, M.E., Scott,J.L, Soni, S.P., Jones, K.R.,  Digman, M.A., Gratton, E., Tessier, C.R. and Stahelin, R.V. Host cell plasma membrane phosphatidylserine regulates the assembly and budding of the Ebola virus. J Virol. 2015

J76.     Mieruszynski,S., Briggs, C., Digman, M.A., Gratton, E. , and Jones, M.R. Live cell characterization of DNA aggregation delivered through lipofection. Sci Rep. 2015; 5: 10528.

J75.     Olivera-Couto, A., Salzman, V., Mailhos, M., Digman, M.A., Gratton, E., and Aguilar, P.S.. Eisosomes are dynamic plasma membrane domains showing Pil1-Lsp1 heteroligomer binding equilibrium. Biophys J. 2015; 108(7): 1633-1644.

J74.     Moens, J.P., Digman, M.A. and Gratton, E.. Modes of diffusion of Cholera toxin bound to GM1 on live cell membrane by image mean square displacement analysis. Biophys J. 2015; 108(6): 1448-1458.

J73.     Chen, H., Gratton, E.,and Digman. M.A. Spectral properties and dynamics of gold nanorods revealed by EMCCD-based spectral phasor method. Microsc Res Tech. 2015; 78(4): 283-293.

2014

J72.     Kang,D., Monsur M., Zhang, K., Huang, S.S., Peterson, E., Digman, M.A., Gratton, E., and Zhao, W. Rapid detection of single bacteria in unprocessed blood using Integrated Comprehensive Droplet Digital Detection. Nat Commun. 2014; 5: 5427. PMCID: PMC4243214

J71.     Adu-Gyamf, E.i, Soni, S.P., Jee, C.L., Digman, M.A., Gratton, E., and Stahelin, R.V.A The loop region in the N-terminal domain of Ebola virus VP40 is important in viral assembly, budding, and egress. Viruses. 2014; 6(10): 3837-3854. PMCID: PMC4213565

J70.     Pate, K.T., Stringari, C., Sprowl Tanio, S., Wang, K,  TeSlaa, T., Hoverter,N.P.,  McQuade, M., Garne, C., Digman, M.A., Teitell, M.A., Edwards, R.A., Gratton, E., Waterman, M.L. “Wnt Signaling Directs a Metabolic Program of Glycolysis and Angiogenesis in Colon Cancer,” The EMBO Journal; May 2014,33(13)1454-1473;DOI: 10.15252/embj.201488598

J69.     Chiu, C., Aguilar, J.S, Tsai, C.Y., Wu, G., E. Gratton and Digman, M.A. ”Nanoimaging of focal adhesions in 3D”, PLOSONE, Published: June 24, 2014 DOI: 10.1371/journal.pone.0099896

J68.     Sharma, H., Digman, M.A., Felsinger, N., Gratton, E. and Khine, M.. “Enhanced emission of fluorophores on shrink-induced wrinkled composite structures.” Optical Materials Express. 2014; 4(4): 753-763.

J67.     Pozzi,D., Marchini, C., Cardarelli,F., Salomone, F., Coppola, S., Montani, M., Zabaleta, M.E.,  Digman, M.A., Gratton, E., Colapicchioni, V., and Caracciolo, G.. “Mechanistic evaluation of the transfection barriers involved in lipid-mediated gene delivery: Interplay between nanostructure and composition.” Biochim Biophys Acta. 2014; 1838(3): 957-967. PMCID: PMC3940149

J66.     James, N.G., Digman, M.A., Ross, J.A., Barylko, B., Wang, L.,  Li,J., Chen, Y., Mueller, J.D., Gratton, E., Albanesi, J.P.,, and Jameson, D.M. “A mutation associated with centronuclear myopathy enhances the size and stability of dynamin 2 complexes in cells.” Biochim Biophys Acta. 2014; 1840(1): 315-321. PMCID: PMC3859711

2013

J65.     Andrews, L.M., Jones, M.R., Digman, M.A., and Gratton, E., Spectral phasor analysis of Pyronin Y labeled RNA microenvironments in living cells. Biomedical Optics Express 2013; 4(1): 171-177.

J64.     Coppola, S., Cardarelli, F., Pozzi, D., Estrada, L.C., Digman, M.A., Gratton, E., Bifone, A., Marianecci, C., and Caracciolo, G., The role of cytoskeleton networks on lipid-mediated delivery of DNA. Ther. Deliv. 2013; Feb. 4(2): 191-202; doi: 10.4155/tde.12.151.

J63.     Chiu, C., Digman, M.A., and Gratton, E., “Cell matrix remodeling ability shown by image spatial correlation”. J Biophys. 2013; 2013, 532030. PMCID: PMC3725897

J62.     Chiu, C., Digman, M.A. and Gratton, E. “Measuring Actin Flow in 3D Cell Protrusion.”  Biophys J. 2013; 105(8): 1746-1755. PMCID: PMC3797595

J61.     Hinde, E. , Digman, M.A., Hahn, K.M., and Gratton, E.”Millisecond spatiotemporal dynamics of FRET biosensors by the pair correlation function and the phasor approach to FLIM.” Proc Natl Acad Sci USA. 2013; 110(1): 135-140. PMCID: PMC3538204

J60.     Adu-Gyamfi, E,  Soni, S.P., Xue, Y., Digman, M.A., Gratton, E. and Stahelin RV.” The Plasma Membrane Induces the Membrane Penetration of the Ebola Virus Matrix Protein: A Key Step in VP40 Oligomerization and Viral Egress.” J Biol Chem. 2013; 288(8): 5779-5789. PMCID: PMC3581432

J59.     Coppola, S., Pozzi, D., Candeloro De Sanctis, S., Digman, M.A., Gratton, E. and Caracciolo, G. “Quantitative measurement of intracellular transport of nanocarriers by spatio-temporal image correlation spectroscopy.” Methods Appl Fluoresc. 2013; 1(1): 015005.

J58.     Andrews, L.M., Jones, M.R., Digman, M.A., and Gratton, E. “Detecting Pyronin Y labeled RNA transcripts in live cell microenvironments by phasor-FLIM analysis.” Methods Appl Fluoresc. 2013; 1(1): 015001

J57.     Torno, K., Wright, B.K., Jones, M.R., Digman, M.A., Gratton, E., and Phillips, M. “Real-time analysis of metabolic activity within lactobacillus acidophilus by phasor fluorescence lifetime imaging microscopy of NADH.” Curr Microbiol. 2013; 66(4): 365-367.

2012

J56.     Adu-Gyamfi E, Digman, M.A., Gratton E, Stahelin RV. “Single-Particle Tracking Demonstrates that Actin Coordinates the Movement of the Ebola Virus Matrix Protein. Biophys J. 2012 Nov 7;103(9):L41-3.

J55.     Wright BK, Andrews LM, Jones MR, Stringari C, Digman M.A., Gratton E. “Phasor-flim analysis of NADH distribution and localization in the nucleus of live progenitor myoblast cells.” Microsc Res Tech. 2012 Dec;75(12):1717-22.

J54.     Whiteside, M.D., Digman, M.A., Grattton, E. and Treseder, K.K. Organic Nitrogen Uptake By Arbuscular Mycorrhizal. Soil Biology & Biochemistry Volume 55, December 2012, Pages 7-13.

J53.     Coppola, S., Estrada, L.C., Digman, MA., Pozzi, P., Cardarelli, F., Gratton, E., and Caracciolo, G. “Intracellular trafficking of cationic liposome–DNA complexes in living cells.”  Soft Matter. 2012; 8(30): 3919-3927

J52.     Wright, B.K., Andrews, L.M., Markham, J., Jones, M.R., Stringari, C., Digman, M.A., and Gratton, E. “NADH distribution in live progenitor stem cells by phasor-fluorescence lifetime image microscopy.” Biophys J. 2012; 103(1): L7-L9

J51.     Adu-Gyamfi, E., Digman, M.A., Gratton, E., and Stahelin, R.V. “Investigation of Ebola VP40 assembly and oligomerization in live cells using number and brightness analysis.”  Biophys J. 2012; 102(11): 2517-2525

J50.     James, N.G., Digman, M.A., Gratton, E., Barylko, B., Ding, X., Albanesi, J.P., Goldberg, M.S. and  Jameson, D.M. “Number and brightness analysis of LRRK2 oligomerization in live cells.” Biophys J. 2012; 102(11): L41-L43

J49.     Battisti, A., Digman, M.A., Gratton, E., Storti, B., Beltram, F., and Bizzarri, R. Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging.  Chem Commun (Camb). 2012; 48(42): 5127-5129.

J48.     Digman, M.A. and  Gratton, E. Scanning image correlation spectroscopy.  Bioessays. 2012; 34(5): 377-385.

J47.     Zhou, S., Lo, W.L., Suhalim, J.L., Digman, M.A., Gratton, E., Nie, Q., and Lander, A.D. Free extracellular diffusion creates the Dpp morphogen gradient of the Drosophila wing disc.  Curr Biol. 2012; 22(8): 668-675.

J46.     Kniazeva, E., Weidling, J.W., Singh, R., Botvinick, E.L., Digman, M.A., Gratton, E., and Putnam, A.J. Quantification of local matrix deformations and mechanical properties during capillary morphogenesis in 3D.  Integr Biol (Camb). 2012; 4(4): 431-439.

J45.     Hinde, E., Digman, M.A., Welch, C., Hahn, K.M. and Gratton, E.. Biosensor Förster resonance energy transfer detection by the phasor approach to fluorescence lifetime imaging microscopy.  Microsc Res Tech. 2012; 75(3): 271-281.

J44.     Hinde, E., Cardarelli, F., Digman, M.A., and Gratton, E.. Changes in chromatin compaction during the cell cycle revealed by micrometer-scale measurement of molecular flow in the nucleus. Biophys J. 2012; 102(3): 691-697.

2011

J43.     Caracciolo, G., Pozzi, D., Capriotti, A.L., Marianecci, C., Carafa, M., Marchini, C., Montani, M. Amici, A., Amenitsch, H. Digman, M.A., Gratton, E., Sanchez, S., and Lagan A., Factors Determining the Superior Performance of Lipid/DNA/Protammine Nanoparticles over Lipoplexes. Journal of Medicinal Chemistry 2011; 54: 4160-4171.

J42.     Marchini, C., Pozzi, D., Montani, M., Alfonsi, C., Amici, A., Candeloro De Sanctis, S, Digman, M.A., Sanchez, S., Gratton, E., Amenitsch, H. Fabbretti, A., Gualerzi, C.O., and Caracciolo, G., Role of temperature-independent lipoplex–cell membrane interactions in the efficiency boost of multicomponent Lipoplexes. Cancer Gene Ther. 2011; 18(8): 543-522; doi: 10.1038/cgt.2011.12.

J41.     Stafford, R.L., Hinde, E., Knight, M.J., Pennella, A., Ear, J., Digman, M.A. Gratton, E.,  Bowie, J.U. “Tandem SAM Domain Structure of Human Caskin1: A Presynaptic, Self-Assembling Scaffold for CASK. “ Structure, 7 December 2011, Vol. 19, Issue 12, pp. 1826-1836.

J40.     Hinde, E., Cardarelli, F., Digman, M.A., and Gratton, E., “The impact of mitotic versus interphase chromatin architecture on the molecular flow of EGFP by pair correlation analysis.” Biophys J. 2011; 100(7): 1829-1836.

J39.     Stringari, C., Cinquin, A., Cinquin, O., Digman, M.A., Donovan, P., and Gratton, E., “Phasor approach to fluorescence lifetime microscopy distinguishes different metabolic states of germ cells in live tissue.” Proc. Natl. Acad. Sci. USA. 2011; 108(33): 13582-13587.

J38.     Lanzano, L., Digman, M.A.,  Fwu, P., Giral H., Levi, M., and Gratton, E., “Nanometer-scale imaging of microvilli in opossum kidney cells by the modulation tracking method.” J Biophotonics 2011; 4(6): 415–424.

J37.     Marchin, C.,Montani, M.,Alfonsi, C, Amici, A.,Digman, M.A., Sanchez, S., Gratton,E., Amenitsch, H.,Pozzi, D.,and Caracciolo, G., “Evidence of a novel mechanism in lipid-mediated gene delivery.” Cancer Gene Ther. 2011; 18(8): 543-552.

J36.     Ross, J.A.,Digman, M.A., Wang, L., Gratton, E., Albanesi, J.P., and Jameson, D.M. “Oligomerization State of Dynamin 2 in Cell Membranes Using TIRF and Number and Brightness Analysis.” Biophys J. 2011; 100(3): L15-17.

J35.     Choi, C.K., Zareno, J.,Digman, M.A.,Gratton, E., and Horwitz, A.R., “Cross-Correlated Fluctuation Analysis Reveals Phosphorylation-Regulated Paxillin-FAK Complexes in Nascent Adhesions” Biophys J. 2011; 100(3): 583-592.

J34.     Vetri, V.,Ossato,G.,Militello, V.,Digman, M.A.,Leone, M., and Gratton, E., “Fluctuation methods to study protein aggregation in live cells: Concanavalin A oligomers formation” Biophys J; Biophys J. 2011; 100(3): 774-783.

2010

J33.     S. A Kim, H. Sanabria, M.A Digman, Enrico Gratton, P. Schwille, W. R. Zipfel, and M. N. Waxham. “Quantifying translational mobility in neurons: comparison between current optical techniques.” J Neurosci. 2010; 30(49): 16409-16416

J32.     Fu, C., Ossato, G., Long, M., Digman, M.A., Gopinathan, A.,Lee, L.P., Gratton, E., Khine, M. “Bimetallic Nanopetals for Thousand-Fold Fluorescence Enhancements.” Applied Physics Letters; 97, 203101 (2010). Cover image.

J31.     Hinde, E., Cardarelli, F., Digman, M.A., and Gratton, E., “In vivo pair correlation analysis of EGFP intranuclear diffusion reveals DNA-dependent molecular flow.” Proc. Natl. Acad. Sci. U S A. 2010 Sep 21;107(38):16560-5. Epub 2010 Sep 7. PMID: 20823232.

J30.     Kuo, W.J., Digman, M.A., Lander, A.D.”Heparan Sulfate Acts as a BMP Co-receptor by Facilitating Ligand-induced Receptor Hetero-oligomerization.” Mol. Biol. Cell. 2010; 21(22): 4028-4041

J29.     D. M Presman, L. D. Alvarez, V. Levi, Silvina E., M. A. Digman, M. A. Martí, A. S. Veleiro, G. Burton, and A. Pecci. “Insights on glucocorticoid receptor activity modulation through the binding of rigid steroids.” PLoS One. 2010; 5(10): e13279

J28.     Rossow, M., Sasaki, J.M., Digman, M.A., and Gratton, E., “Raster image correlation spectroscopy in live cells.” Nature Protocols, V5, P1761–1774, doi:10.1038/nprot.2010.122.

J27.     Ossato G., Digman M.A., Aiken C., Lukacsovich T., Marsh J.L., Gratton E. “A two step path to inclusion formation of Huntingtin peptides revealed by N&B analysis.” Biophys J. 2010 Jun 16;98(12):3078-85. PMID: 20550921.

J26.     Yoon, Y., Tong, J., Lee, P.J., Albanese, A., Bhardwaj, N., Källberg. M., Digman, M.A., Lu, H., Gratton, E., Shin, Y., Cho, W. “Molecular basis of the potent membrane remodeling activity of the epsin1 ENTH domain.” J. Biol. Chem. 2010; 285(1): 531-540. PMCID: PMC2804201.

2009

J25.     Digman, M.A. and Gratton E. “Imaging barriers to diffusion by pair correlation functions.” Biophys J. 2009; 97(2): 665-673. PMCID: PMC2711318.

J24.     Digman, M.A., Wiseman. P.W., Choi, C., Horwitz, A.R., Gratton, E. “Mapping the stoichiometry of molecular complexes at focal adhesions in live cells.” Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2170-5. PMCID: 2630200.

J23.     Digman, M.A., Wiseman. P.W., Horwitz, A.R., Gratton, E. “Mapping protein complexes in living cells from laser scanning confocal image sequences: the ccRICS method.” Biophys J. 2009 Jan;96(2): 707-16.

J22.     M.A. Digman and E. Gratton.”Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy, applications in systems biology.” WIREs Syst Biol Med. 2009; 1(2): 273-282.

J21.     Caracciolo, G., Caminiti, R., Digman, M.A., Gratton, E., Sanchez, S. “Efficient escape from endosomes determines the superior efficiency of multicomponent lipoplexes,”J Phys Chem B. 2009 Apr 16;113(15):4995-7.

J20.     Vetri V., Ossato G., Militello V., Digman, M.A., Leone M., and Gratton E. “Oligomerization of Concanavalin A in live cells detected by fluctuation analysis.” Eur Biophys J. 2009; 38(Suppl 1): S137, P-393.

J19.     Gielen E, Smisdom N, Vandeven M, De Clercq B, Gratton E, Digman M., Rigo JM, Hofkens J, Engelborghs Y, Ameloot M. “Measuring Diffusion of Lipid-like Probes in Artificial and Natural Membranes by Raster Image Correlation Spectroscopy (RICS): Use of a Commercial Laser-Scanning Microscope with Analog Detection.”  Langmuir. 2009 May 5;25(9): 5209-18.

2008

J18.     Digman, M.A., and Gratton, E. “Analysis of diffusion and binding in cells using the RICS approach.” Microscopy Res. Tech. 2008; Dec 9;72(4): 323-332. PMID: 19067357.

J17.     Watson NB, Nelson E, Digman M., Thornburg JA, Alphenaar BW, McGregor WG. “RAD18 and associated proteins are immobilized in nuclear foci in human cells entering S-phase with ultraviolet light-induced damage.” Mutat Res. 2008 15;648(1-2): 23-31.

J16.     Sanabria, H., Digman, M.A., Gratton E., and Waxham M.N. “Spatial Diffusivity and Availability of Intracellular Calmodulin” Biophys J. 2008 Dec 15;95(12): 6002-15.

J15.     C.M. Brown, Dalal, R.,Hebert, B., Digman, M.A., Gratton, E., Horwitz, A.F., “Raster Image Correlation Spectroscopy (RICS) for Measuring Fast Protein Dynamics and Concentrations with a Commercial Laser Scanning Confocal Microscope.” J Microsc. 2008 Jan;229(Pt 1): 78-91.

J14.     Digman, M.A., R. Dalal, C.M. Brown, A.F. Horwitz,  E. Gratton, “Mapping the number     of molecules and brightness in the laser scanning microscope.” Biophys J. 2008 Mar 15;94(6): 2320-32.

J13.     Digman M.A., C.M. Brown, A. F. Horwitz, W.W. Mantulin, and E. Gratton. “Paxillin dynamics measured during adhesion assembly and disassembly by correlation spectroscopy.” Biophys J.  Apr 1;94(7): 2819-31, 2008.

J12.     Digman M.A., V. R. Caiolfa, M. Zamai, and E. Gratton. “The Phasor approach to fluorescence lifetime imaging analysis.” Biophys J. 2008 Jan 15;94(2): L14-6.

J11.     Dalal, R.B., M. A. Digman, A. F. Horwitz, V. Vetri, and E. Gratton. “Determination of particle number and brightness using a laser scanning confocal microscope operating in the analog mode. Microsc. Res. Tech. 2008 Jan;71(1): 69-81.

2007

J10.     Caiolfa V.R., M. Zamai, G. Malengo, A. Andolfo, C. D. Madsen, J. D. B. Sutin, M. A. Digman, E. Gratton, F. Blasi, and N. Sidenius. “Monomer–dimer dynamics and distribution of GPI-anchored uPAR are determined by cell surface protein assemblies.”  J Cell Biol 2007 179(5): 1067-1082.

J9.       Gokhale NA, A. Abraham, M.A. Digman, E. Gratton, and W. Cho. “Phosphoinositide specificity of and mechanism of lipid domain formation by annexin A2-p11 heterotetramer.” J. Biol. Chem. 2005, 280(52): 42831-40.

2005

J8.       Digman M.A., C.M. Brown , P. Sengupta , P.W. Wiseman, A.R. Horwitz, and E. Gratton E. “Measuring fast dynamics in solutions and cells with a laser scanning microscope.” Biophys. J. 2005 Aug;89(2): 1317-27.

J7.       Digman M.A., P. Sengupta, P.W. Wiseman, C.M. Brown, A.R. Horwitz, and E. Gratton. “Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure.” Biophys. J. 2005 May;88(5): L33-6.

J6.       Stahelin R.V., M.A. Digman, M. Medkova, B. Ananthanarayanan, H.R. Melowic, J.D. Rafter, and W. Cho. “Diacylglycerol-induced membrane targeting and activation of protein kinase Cepsilon: mechanistic differences between protein kinases Cdelta and Cepsilon.” J Biol Chem. 2005 May 20;280(20): 19784-93.

2004

J5.       Barcellona M.L., S. Gammon, T. Hazlett, M.A. Digman, and E. Gratton. “Polarized fluorescence correlation spectroscopy of DNA-DAPI complexes.” Microsc. Res. Tech. 2004 Nov; 65(4-5): 205-17.

J4.       Inoue M., M.A. Digman, M. Cheng, S.Y. Breusegem, N. Halaihel, V. Sorribas, W.W. Mantulin, E. Gratton, N.P. Barry, and M. Levi. “Partitioning of NaPi cotransporter in cholesterol-, sphingomyelin-, and glycosphingolipid-enriched membrane domains modulates NaPi protein diffusion, clustering, and activity.” J. Biol. Chem. 2004 Nov 19;279(47): 49160-71.

J3.       Stahelin R.V., M.A. Digman, M. Medkova, B. Ananthanarayanan, J.D. Rafter, H. Melowic, and W. Cho. “Mechanism of diacylglycerol-induced membrane targeting and activation of proteinkinase Cdelta.” J. Biol. Chem. 2004 Jul 9;279(28): 29501-12.

2003

J2.       Ananthanarayanan B., R.V. Stahelin , M.A. Digman, W. Cho, “Activation mechanisms of conventional protein kinase C isoforms are determined by the ligand affinity and conformational flexibility of their C1 domains.” J. Biol. Chem. 2003 Nov 21;278(47): 46886-94.

2000

J1.       Narducci, A.A., Yang, Y., Digman, M.A., Sipes, A.B., Ibers, J.A., “An investigation of the rare-earth telluride system BaLn2Te4 (Ln=Sm–Tm, Y): syntheses, crystal structures, and magnetic properties. Journal of Alloys and Compounds, 2000 May 24, 303-304: 432-439.