J 131. Sohrabi A, Lefebvre AEYT, Harrison MJ, Condro MC, Sanazzaro TM, Safarians G, Solomon I, Bastola S, Kordbacheh S, Toh N, Kornblum HI, Digman MA, Seidlits SK. Cell Rep. 2023 Sep 26;42(10):113175. doi: 10.1016/j.celrep.2023.113175. Epub ahead of print. PMID: 37756163.
J 130. Fazel M, Vallmitjana A, Scipioni L, Gratton E, Digman MA, Pressé S. Fluorescence lifetime: Beating the IRF and interpulse window. Biophys J. 2023; 122(4): 672-683. PMCID: PMC9989884
2022
J129. Fang T, Huang YK, Wei J, Mena JEM, Lakey PSJ, Kleinman MT, Digman MA, Shiraiwa M. Superoxide Release by Macrophages through NADPH Oxidase Activation Dominating Chemistry by Isoprene Secondary Organic Aerosols and Quinones to Cause Oxidative Damage on Membranes. Environ Sci Technol. 2022; 56(23): 17029-17038. PMCID: PMC9730850
J128. Halbrook CJ, Thurston G, Boyer S, Anaraki C, Jiménez JA, McCarthy A, Steele NG, Kerk SA, Hong HS, Lin L, Law FV, Felton C, Scipioni L, Sajjakulnukit P, Andren A, Beutel AK, Singh R, Nelson BS, van Bergh FD, Krall AS, Mullen PJ, Zhang L, Batra S, Morton JP, Stanger BZ, Christofk HR, Digman MA, Beard DA, Viale A, Zhang J, Crawford HC, di Magliano MP, Jorgensen C, Lyssiotis CA. Differential integrated stress response and asparagine production drive symbiosis and therapy resistance of pancreatic adenocarcinoma cells. Nat Cancer. 2022; 3(11): 1386-1403. PMCID: PMC9701142
J127. Chun SK, Fortin BM, Fellows RC, Habowski AN, Verlande A, Song WA, Mahieu AL, Lefebvre AEYT, Sterrenberg JN, Velez LM, Digman MA, Edwards RA, Pannunzio NR, Seldin MM, Waterman ML, Masri S. Disruption of the circadian clock drives Apc loss of heterozygosity to accelerate colorectal cancer. Sci Adv. 2022; 8(32), eabo2389. PMCID: PMC9365282
J126. Yao Z, Brennan CK, Scipioni L, Chen H, Ng KK, Tedeschi G, Parag-Sharma K, Amelio AL, Gratton E, Digman* MA, Prescher* JA. Multiplexed bioluminescence microscopy via phasor analysis. Nat Methods. 2022; 19(7): 893-898. (*co-corresponding authors)
J125. Fazel M, Jazani S, Scipioni L, Vallmitjana A, Gratton E, Digman MA, Pressé S. High resolution fluorescence lifetime maps from minimal photon counts. ACS Photonics. 2022; 9(3): 1015-1025. PMCID: PMC9278809
2021
J124. Ma D, Hernandez GA, Lefebvre AEYT, Alshetaiwi H, Blake K, Dave KR, Rauf M, Williams JW, Davis RT, Evans KT, Longworth A, Masoud MYG, Lee R, Edwards RA, Digman MA, Kessenbrock K, Lawson DA. Patient-derived xenograft culture-transplant system for investigation of human breast cancer metastasis.Commun Biol. 2021; 4(1): 1268. PMCID: PMC8571269
J123. Shi B, Li W, Song Y, Wang Z, Ju R, Ulman A, Hu J, Palomba F, Zhao Y, Le JP, Jarrard W, Dimoff D, Digman MA, Gratton E, Zang C, Jiang H. UTX condensation underlies its tumour-suppressive activity.Nature. 2021; 597(7878): 726-731. PMCID: PMC9008583
J-122. Li W, Hu J, Shi B, Palomba F, Digman MA, Gratton E, Jiang H. Biophysical properties of AKAP95 protein condensates regulate splicing and tumorigenesis. Nat Cell Biol. 2020;22(8):960-972. doi:10.1038/s41556-020-0550-8
J121. Lefebvre AEYT, Ma D, Kessenbrock K, Lawson DA, Digman MA.Automated segmentation and tracking of mitochondria in live-cell time-lapse images.Nat Methods. 2021; 18(9): 1091-1102.
J120. Tedeschi G, Scipioni L, Papanikolaou M, Abbott GW, Digman MA. Fluorescence Fluctuation Spectroscopy enables quantification of potassium channel subunit dynamics and stoichiometry. Sci Rep. 2021; 11(1), 10719. PMCID: PMC8140153
2020
J119. Makaremi S, Rose M, Ranjit S, Digman MA, Bowdish DME, Moran-Mirabal JM. Lateral diffusion of CD14 and TLR2 in macrophage plasma membrane assessed by raster image correlation spectroscopy and single particle tracking. Sci Rep. 2020; 10(1): 19375. PMCID: PMC7652837
J118. Li W, Hu J, Shi B, Palomba F, Digman MA, Gratton E, Jiang H. Biophysical properties of AKAP95 protein condensates regulate splicing and tumorigenesis. Nat Cell Biol. 2020;22(8):960-972. doi:10.1038/s41556-020-0550-8
J117. Sameni S, Zhang R, Digman MA.The phasor FLIM method reveals a link between a change in energy metabolism and mHtt protein spread in healthy mammalian cells when co-cultured with Huntington diseased cells. Methods Appl Fluoresc. 2021; 9(1), 015005.
J116. Pascua SM, McGahey GE, Ma N, Wang JJ, Digman MA. Caffeine and Cisplatin effectively targets the metabolism of a triple-negative breast cancer cell line assessed via Phasor-FLIM. Int J Mol Sci. 2020; 21(7), E2443. PMCID: PMC7177700
J115. Perinbam K, Chacko, J., Kannan A, Digman MA, Siryaporn A. A shift in central metabolism accompanies virulence activation in Pseudomonas aeruginosa. mBio. 2020; 11(2), e02730-18.
J114. Davis RT, Blake K, Ma D, Gabra MBI, Hernandez GA, Phung AT, Yang Y, Maurer D, Lefebvre AEYT, Alshetaiwi H, Xiao Z, Liu J, Locasale JW, Digman MA, Mjolsness E, Kong M, Werb Z, Lawson DA. Transcriptional diversity and bioenergetic shift in human breast cancer metastasis revealed by single-cell RNA sequencing. Nat Cell Biol. 2020; 22(3): 310-320.
J113. Sameni S, Zhang R, Digman MA. Number and molecular brightness analysis reveals Htt25Q protein aggregation upon the uptake of Htt97Q aggregates.
Biochem Biophys Res Commun. 2020; 522(1): 133-137.
2019
J112. Trivedi P, Palomba F, Niedzialkowska E, Digman MA, Gratton E, Stukenberg PT. The inner centromere is a biomolecular condensate scaffolded by the chromosomal passenger complex. Nat Cell Biol. 2019 Sep;21(9):1127-1137. doi: 10.1038/s41556-019-0376-4. Epub 2019 Sep 3.
J111. Murata MM, Kong X, Moncada E, Chen Y, Imamura H, Wang P, Berns MW, Yokomori K, Digman MA. NAD+ consumption by PARP1 in response to DNA damage triggers metabolic shift critical for damaged cell survival. Mol Biol Cell. 2019; 30(20): 2584-2597. PMCID: PMC6740200
J110. Ma N, de Mochel NR, Pham PD, Yoo TY, Cho KWY, Digman MA. Label-free assessment of pre-implantation embryo quality by the Fluorescence Lifetime Imaging Microscopy (FLIM)-phasor approach. Sci Rep. 2019; 9(1), 13206. PMCID: PMC6744410
J109. Dong Y, Sameni S, Digman MA, Brewer GJ. Reversibility of age-related oxidized free NADH redox states in Alzheimer’s disease neurons by imposed external Cys/CySS redox shifts. Sci Rep. 2019; 9(1), 11274. PMCID: PMC6677822
J108. Chen H, Ma N, Kagawa K, Kawahito S, Digman M, Gratton E. Widefield multifrequency fluorescence lifetime imaging using a two-tap complementary metal-oxide semiconductor camera with lateral electric field charge modulators. J.Biophotonics. 2019; 12(5), e201800223.
J107. Planes N, Digman MA, Vanderheyden PPML, Gratton E, Caballero-George C. Number and brightness analysis to study spatio-temporal distribution of the angiotensin II AT1 and the endothelin-1 ETA receptors: Influence of ligand binding. Biochim Biophys Acta Gen Subj. 2019; 1863(5): 917-924.
J106. Dong Y, Digman MA, Brewer GJ. Age- and AD-related redox state of NADH in subcellular compartments by fluorescence lifetime imaging microscopy. Geroscience. 2019; 41(1): 51-67. PMCID: PMC6423217
J105. Ma N, Kamalakshakurup G, Aghaamoo M, Lee AP, Digman MA. Label-Free Metabolic Classification of Single Cells in Droplets Using the Phasor Approach to Fluorescence Lifetime Imaging Microscopy. Cytometry A. 2019; 95(1): 93-100.
2018
J104. Mah EJ, Lefebvre AEYT, McGahey GE, Yee AF, Digman MA. Collagen density modulates triple-negative breast cancer cell metabolism through adhesion-mediated contractility. Sci Rep. 2018 Nov 20;8(1):17094. doi: 10.1038/s41598-018-35381-9.
J103. Anzalone A, Chacko JV, Nishi RA, Dumont C, Smith D, Shea LD, Digman MA, Cummings BJ, Anderson AJ. Feasibility study on mouse live imaging after spinal cord injury and poly(lactide-co-glycolide) bridge implantation. J Biomed Opt. 2018 Jun;23(6):1-6. doi: 10.1117/1.JBO.23.6.065007
J102. Kim SM, Nguyen TT, Ravi A, Kubiniok P, Finicle BT, Jayashankar V, Malacrida L, Hou J, Robertson J, Gao D, Chernoff J, Digman MA, Potma EO, Tromberg BJ, Thibault P, Edinger AL. PTEN deficiency and AMPK activation promote nutrient scavenging and anabolism in prostate cancer cells. Cancer Discov. 2018; 8(7): 866-883. PMCID: PMC6030497
J101. Mäntylä E, Chacko JV, Aho V, Parrish CR, Shahin V, Kann M, Digman MA, Gratton E, Vihinen-Ranta M. Viral highway to nucleus exposed by image correlation analyses.
Sci Rep. 2018 Jan 18;8(1):1152. doi: 10.1038/s41598-018-19582-w.
J100. Lee DH, Li X, Ma N, Digman MA, Lee AP. Rapid and label-free identification of single leukemia cells from blood in a high-density microfluidic trapping array by fluorescence lifetime imaging microscopy. Lab Chip. 2018 May 1;18(9):1349-1358. doi: 10.1039/c7lc01301a.
J99. Kong, L., Murata, M. M. and Digman, M. A. Absence of REV3L promotes p53-regulated cancer cell metabolism in cisplatin-treated lung carcinoma cells. Biochem Biophys Res Commun 496, 199-204, doi:10.1016/j.bbrc.2018.01.026 (2018).
J98. Sameni, S., Malacrida, L., Tan, Z.and Digman, M. A. Alteration in Fluidity of Cell Plasma Membrane in Huntington Disease Revealed by Spectral Phasor Analysis. Scientific Reports 8, 734, doi:10.1038/s41598-018-19160-0 (2018).
2017
J97. Trinh, A., Chen,H., Chen, y., Hu, Y. Li, Z., Siegel, E.R., Linskey, M.E., Wang, P.W., Digman*, M.A. and Zhou*, Y., “Tracking Functional Tumor Cell Subpopulations of Malignant Glioma by Phasor Fluorescence Lifetime Imaging Microscopy of NADH”, Y. Cancers 2017, 9(12), 168; doi:10.3390/cancers9120168 *co-corresponding authors
J96. Davey RJ, Digman MA, Gratton E, Moens PDJ. Quantitative image mean squared displacement (iMSD) analysis of the dynamics of profilin 1 at the membrane of live cells. Methods. 2018 May 1;140-141:119-125. doi: 10.1016/j.ymeth.2017.12.002. Epub 2017 Dec 11.PMID:29242135
J95. Digiacomo L, Cardarelli F, Pozzi D, Palchetti S, Digman MA, Gratton E, Capriotti AL, Mahmoudi M, Caracciolo G. An apolipoprotein-enriched biomolecular corona switches the cellular uptake mechanism and trafficking pathway of lipid nanoparticles. Nanoscale. 2017; 9(44): 17254-17262. PMCID: PMC5700750
J94. Liu L, Zhang SX, Liao W, Farhoodi HP, Wong CW, Chen CC, Ségaliny AI, Chacko JV, Nguyen LP, Lu M, Polovin G, Pone EJ, Downing TL, Lawson DA, Digman MA, Zhao W. Mechanoresponsive stem cells to target cancer metastases through biophysical cues. Sci Transl Med. 2017; 9(400), aan2966.
J93. Herrington KA, Trinh AL, Dang C, O’Shaughnessy E, Hahn KM, Gratton E, Digman MA, Sütterlin C. Spatial analysis of Cdc42 activity reveals a role for plasma membrane-associated Cdc42 in centrosome regulation. Mol Biol Cell. 2017 Jul 15;28(15):2135-2145. doi: 10.1091/mbc.E16-09-0665. Epub 2017 May 24.PMID:28539409.
J92. Liang EI, Mah EJ, Yee AF, Digman MA. Correlation of focal adhesion assembly and disassembly with cell migration on nanotopography. Integr Biol (Camb). 2017; 9(2): 145-155. PMCID: PMC5399776
J91. de Vega GB, Ceballos JA, Anzalone A, Digman MA, Gratton E. A laser-scanning confocal microscopy study of carrageenan in red algae from seaweed farms near the Caribbean entrance of the Panama Canal. J Appl Phycol. 2017; 29(1): 495-508.
J90. Romero-López M, Trinh AL, Sobrino A, Hatch MMS, Keating MT, Fimbres C, Lewis DE, Gershon PD, Botvinick EL, Digman MA, Lowengrub JS, Hughes CCW. Recapitulating the human tumor microenvironment: Colon tumor-derived extracellular matrix promotes angiogenesis and tumor cell growth. Biomaterials. 2017; 116: 118-129. PMCID: PMC5226635
2016
J89. Qiu, Y., Lin, C., Hinkle, P., Plett, T.S., Yang, C., Varghese Chacko, J. Digman, M.A., Yeh, L., Hsu, J. and Siwy, Z., Highly Charged Particles Cause a Larger Current Blockage in Micropores Compared to Neutral Particles. , American Chemical Society, ACS Nao 2016; 10: 8413-8422. doi: 10.1021/acsnano.6b03280
J88. Sameni, A., Syed, A., Marsh, J.L., and Digman, M.A., The phasor-FLIM fingerprints reveal shifts from OXPHOS to enhanced glycolysis in Huntington Disease. Scientific Reports 6, Article number: 34755 (2016); doi:10.1038/srep34755.
J87. Cinco,R., Digman, M.A., Gratton, E. and Luderer, U. Spatial characterization of bioenergetics and metabolism of primordial to preovulatory follicles in whole ex vivo murine ovary. Biol Reprod. 95(6):129. doi: 10.1095/biolreprod.116.142141. Epub 2016 Sep 28.
J86. Digiacomo, L., Digman, M.A., Gratton, E., and Caracciolo, G. Development of an image Mean Square Displacement (iMSD)-based method as a novel approach to study the intracellular trafficking of nanoparticles. Acta Biomater. 2016; 42: 189-198.
J85. Batista de Vega, G., Ceballos, J.A., Anzalone, A., Digman, M.A. and Gratton, E. A laser-scanning confocal microscopy study of carrageenan in red algae from seaweed farms near the Caribbean entrance of the Panama Canal. J Appl Phycol (2017) 29: 495. https://doi.org/10.1007/s10811-016-0914-4.
J84. Chen, H., Gratton, E. and Digman, M.A. Self-assisted optothermal trapping of gold nanorods under two-photon excitation, Methods Appl Fluoresc. 2016; 4(3): 035003. PMCID: PMC5497913
J83. Ma, N., Digman, M.A., Malacrida,L. and Gratton, E. Measurements of absolute concentrations of NADH in cells using the phasor FLIM method. Biomed. Opt. Express 7(7), 2441-2452 (2016)
J82. Sosnik, J., Zheng, L., Rackauckas, C.V., Digman, M.A., Gratton, E., Nie, Q., and Schilling, T. Noise modulation in retinoic acid signaling sharpens segmental boundaries of gene expression in the embryonic zebrafish hindbrain. ELife. 2016; 5: e14034. PMCID: PMC4829421
2015
J81. Hendrix, J., Baumgärtel, V., Schrimpf, W., Ivanchenko, S., Digman, M.A., Gratton, E., Kräusslich, H., Müller, B., and Lamb, D., Live-cell observation of cytosolic HIV-1 assembly onset reveals RNA-interacting Gag oligomers. J. Cell Biol. 2015; 210(4): 629-626. doi: 10.1083/jcb.201504006.
J80. Mieruszynski, S., Digman, M.A., Gratton, E., and Jones, M.R., Characterization of exogenous DNA mobility in live cells through fluctuation correlation spectroscopy. Scientific Reports 5, Article 13848 (2015); doi: 10.1038/srep13848
J79. Kaixiang Zhang, Dong-Ku Kang, M Monsur Ali, Linan Liu, Louai Labanieh, Mengrou Lu, Hamidreza Riazifar, Thi N Nguyen, Jason A Zell, Michelle A Digman, Enrico Gratton, Jinghong Li, and Weian Zhao. Digital quantification of miRNA directly in plasma using integrated comprehensive droplet digital detection. Lab Chip. 2015; 15(21): 4217-4226. PMCID: PMC4631652
J78. Z Tan, W Dai, T G M van Erp, J Overman, A Demuro, Michelle A Digman, A Hatami, R Albay, E M Sontag, K T Potkin, S Ling, F Macciardi, W E Bunney, J D Long, J S Paulsen, J M Ringman, I Parker, C Glabe, L M Thompson, W Chiu, and S G Potkin. Huntington’s disease cerebrospinal fluid seeds aggregation of mutant huntingtin.Mol Psychiatry. 2015; 20(11): 1286-1293.
J77. Adu-Gyamfi, E., Johnson, K.A., Fraser, M.E., Scott,J.L, Soni, S.P., Jones, K.R., Digman, M.A., Gratton, E., Tessier, C.R. and Stahelin, R.V. Host cell plasma membrane phosphatidylserine regulates the assembly and budding of the Ebola virus. J Virol. 2015
J76. Mieruszynski,S., Briggs, C., Digman, M.A., Gratton, E. , and Jones, M.R. Live cell characterization of DNA aggregation delivered through lipofection. Sci Rep. 2015; 5: 10528.
J75. Olivera-Couto, A., Salzman, V., Mailhos, M., Digman, M.A., Gratton, E., and Aguilar, P.S.. Eisosomes are dynamic plasma membrane domains showing Pil1-Lsp1 heteroligomer binding equilibrium. Biophys J. 2015; 108(7): 1633-1644.
J74. Moens, J.P., Digman, M.A. and Gratton, E.. Modes of diffusion of Cholera toxin bound to GM1 on live cell membrane by image mean square displacement analysis. Biophys J. 2015; 108(6): 1448-1458.
J73. Chen, H., Gratton, E.,and Digman. M.A. Spectral properties and dynamics of gold nanorods revealed by EMCCD-based spectral phasor method. Microsc Res Tech. 2015; 78(4): 283-293.
2014
J72. Kang,D., Monsur M., Zhang, K., Huang, S.S., Peterson, E., Digman, M.A., Gratton, E., and Zhao, W. Rapid detection of single bacteria in unprocessed blood using Integrated Comprehensive Droplet Digital Detection. Nat Commun. 2014; 5: 5427. PMCID: PMC4243214
J71. Adu-Gyamf, E.i, Soni, S.P., Jee, C.L., Digman, M.A., Gratton, E., and Stahelin, R.V.A The loop region in the N-terminal domain of Ebola virus VP40 is important in viral assembly, budding, and egress. Viruses. 2014; 6(10): 3837-3854. PMCID: PMC4213565
J70. Pate, K.T., Stringari, C., Sprowl Tanio, S., Wang, K, TeSlaa, T., Hoverter,N.P., McQuade, M., Garne, C., Digman, M.A., Teitell, M.A., Edwards, R.A., Gratton, E., Waterman, M.L. “Wnt Signaling Directs a Metabolic Program of Glycolysis and Angiogenesis in Colon Cancer,” The EMBO Journal; May 2014,33(13)1454-1473;DOI: 10.15252/embj.201488598
J69. Chiu, C., Aguilar, J.S, Tsai, C.Y., Wu, G., E. Gratton and Digman, M.A. ”Nanoimaging of focal adhesions in 3D”, PLOSONE, Published: June 24, 2014 DOI: 10.1371/journal.pone.0099896
J68. Sharma, H., Digman, M.A., Felsinger, N., Gratton, E. and Khine, M.. “Enhanced emission of fluorophores on shrink-induced wrinkled composite structures.” Optical Materials Express. 2014; 4(4): 753-763.
J67. Pozzi,D., Marchini, C., Cardarelli,F., Salomone, F., Coppola, S., Montani, M., Zabaleta, M.E., Digman, M.A., Gratton, E., Colapicchioni, V., and Caracciolo, G.. “Mechanistic evaluation of the transfection barriers involved in lipid-mediated gene delivery: Interplay between nanostructure and composition.” Biochim Biophys Acta. 2014; 1838(3): 957-967. PMCID: PMC3940149
J66. James, N.G., Digman, M.A., Ross, J.A., Barylko, B., Wang, L., Li,J., Chen, Y., Mueller, J.D., Gratton, E., Albanesi, J.P.,, and Jameson, D.M. “A mutation associated with centronuclear myopathy enhances the size and stability of dynamin 2 complexes in cells.” Biochim Biophys Acta. 2014; 1840(1): 315-321. PMCID: PMC3859711
2013
J65. Andrews, L.M., Jones, M.R., Digman, M.A., and Gratton, E., Spectral phasor analysis of Pyronin Y labeled RNA microenvironments in living cells. Biomedical Optics Express 2013; 4(1): 171-177.
J64. Coppola, S., Cardarelli, F., Pozzi, D., Estrada, L.C., Digman, M.A., Gratton, E., Bifone, A., Marianecci, C., and Caracciolo, G., The role of cytoskeleton networks on lipid-mediated delivery of DNA. Ther. Deliv. 2013; Feb. 4(2): 191-202; doi: 10.4155/tde.12.151.
J63. Chiu, C., Digman, M.A., and Gratton, E., “Cell matrix remodeling ability shown by image spatial correlation”. J Biophys. 2013; 2013, 532030. PMCID: PMC3725897
J62. Chiu, C., Digman, M.A. and Gratton, E. “Measuring Actin Flow in 3D Cell Protrusion.” Biophys J. 2013; 105(8): 1746-1755. PMCID: PMC3797595
J61. Hinde, E. , Digman, M.A., Hahn, K.M., and Gratton, E.”Millisecond spatiotemporal dynamics of FRET biosensors by the pair correlation function and the phasor approach to FLIM.” Proc Natl Acad Sci USA. 2013; 110(1): 135-140. PMCID: PMC3538204
J60. Adu-Gyamfi, E, Soni, S.P., Xue, Y., Digman, M.A., Gratton, E. and Stahelin RV.” The Plasma Membrane Induces the Membrane Penetration of the Ebola Virus Matrix Protein: A Key Step in VP40 Oligomerization and Viral Egress.” J Biol Chem. 2013; 288(8): 5779-5789. PMCID: PMC3581432
J59. Coppola, S., Pozzi, D., Candeloro De Sanctis, S., Digman, M.A., Gratton, E. and Caracciolo, G. “Quantitative measurement of intracellular transport of nanocarriers by spatio-temporal image correlation spectroscopy.” Methods Appl Fluoresc. 2013; 1(1): 015005.
J58. Andrews, L.M., Jones, M.R., Digman, M.A., and Gratton, E. “Detecting Pyronin Y labeled RNA transcripts in live cell microenvironments by phasor-FLIM analysis.” Methods Appl Fluoresc. 2013; 1(1): 015001
J57. Torno, K., Wright, B.K., Jones, M.R., Digman, M.A., Gratton, E., and Phillips, M. “Real-time analysis of metabolic activity within lactobacillus acidophilus by phasor fluorescence lifetime imaging microscopy of NADH.” Curr Microbiol. 2013; 66(4): 365-367.